PAMPA study of the temperature effect on permeability

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Gábor VizserálekBálint Sinkó

Abstract

The purpose of this work was to investigate the temperature dependence of permeability measured by PAMPA method. The effective permeability (logPe) of seven drugs representing diverse structures and different acid-base properties was determined on three membrane models (GIT, BBB, Skin). The incubation temperature was varied in the range of 15-55 °C with ten degree steps. The intrinsic permeability (logP0) of the compounds is in linear relation with temperature (T). The slope of the logP0=aT+b regression equation is a good measure of the temperature effect on permeability. Results show intensive and significant temperature dependence of permeability influenced by the properties of the compounds and also by the selected PAMPA model. The Skin PAMPA(™) proved to be the most sensitive on temperature alteration, though GIT and BBB PAMPA results were also affected. The compound with acid function showed the lowest temperature dependence, while the permeability of bases increased considerably in response to the increasing temperature. The importance of human-relevant incubation conditions at in vitro assays is concluded for the better in vivo prediction.

References

Oct 24, 2001·European Journal of Medicinal Chemistry·J A PlattsD Butina
May 15, 2002·European Journal of Medicinal Chemistry·Chengyue ZhuKin-Kai Hwang
Apr 2, 2003·European Journal of Medicinal Chemistry·Li DiGuy T Carter
Apr 25, 2003·International Journal of Pharmaceutics·Kiyohiko SuganoYoshinori Aso
Mar 1, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Alex AvdeefStaffan Tavelin
Jun 20, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Paul R SeoJames E Polli
Jul 13, 2007·International Journal of Pharmaceutics·Simone CarraraChiara Bigogno
Sep 7, 2007·Journal of Pharmaceutical Sciences·Alex AvdeefYukinori Yamauchi
Jan 11, 2008·Pharmaceutical Research·Xiaoxi ChenPraveen V Balimane
Mar 4, 2008·Journal of Medicinal Chemistry·Christopher W CunninghamAndrew Coop
Nov 26, 2009·Chemistry & Biodiversity·Bálint SinkóKrisztina Takács-Novák
Jan 14, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Jurgen MenschPatrick Augustijns
May 5, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Mario A QuevedoMargarita C Briñón
Feb 14, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Bálint SinkóKrisztina Takács-Novák

❮ Previous
Next ❯

Citations

Sep 8, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Judit MüllerGyörgy T Balogh
Jul 9, 2016·Biomaterials·Juan Aparicio-BlancoAna-Isabel Torres-Suárez
Feb 14, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Bálint SinkóKrisztina Takács-Novák
Aug 8, 2014·Pharmaceutical Research·Christopher A PalmiottiLuca Cucullo
Sep 4, 2019·Journal of Computational Chemistry·Hong ZhangChristophe Chipot
Jul 3, 2019·International Journal of Molecular Sciences·Cheng-Ting ChiMax K Leong
Jul 22, 2021·The Journal of Physical Chemistry. B·Christopher Faulkner, Nora H de Leeuw

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Related Papers

Acta pharmaceutica Hungarica
Bálint Sinkó, Krisztina Takácsné Novák
European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Jeffrey A RuellAlex Avdeef
European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Giovanna CortiPaola Mura
© 2021 Meta ULC. All rights reserved