Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

Journal of Clinical Microbiology
Alyssa K Van DreumelGrantley R Peck

Abstract

An amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described. The amino acid consensus sequence of the 3B protein was determined from a multiple-sequence alignment of 125 sequences of 3B. The consensus 3B (c3B) protein was expressed as a soluble recombinant fusion protein with maltose-binding protein (MBP) using a bacterial expression system and was affinity purified using amylose resin. The MBP-c3B protein was used as the antigen in the development of a competition enzyme-linked immunosorbent assay (cELISA) for detection of anti-3B antibodies in bovine sera. The comparative diagnostic sensitivity and specificity at 47% inhibition were estimated to be 87.22% and 93.15%, respectively. Reactivity of c3B with bovine sera representing the seven FMDV serotypes demonstrated the pan-serotype diagnostic capability of this bioreagent. The consensus antigen and competition ELISA are described here as candidates for a pan-serotype diagnostic test for FMDV infection.

References

Sep 2, 1985·FEBS Letters·G W WellingS Welling-Wester
Aug 1, 1997·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·M Grzybowski, J G Younger
Mar 7, 2002·Journal of Virological Methods·Brian J ShiellWojtek P Michalski
Oct 9, 2002·Comparative Immunology, Microbiology and Infectious Diseases·Michelle RémondFrançoise Lebreton
Oct 9, 2002·Comparative Immunology, Microbiology and Infectious Diseases·Gareth Davies
Oct 9, 2002·Comparative Immunology, Microbiology and Infectious Diseases·Yves LeforbanMark Rweyemamu
Jul 16, 2003·Journal of Comparative Pathology·S AlexandersenA J M Garland
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jan 1, 1950·Cancer·W J YOUDEN
Jan 15, 2005·Current Topics in Microbiology and Immunology·S Alexandersen, N Mowat
Jan 22, 2005·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Apr 12, 2005·Preventive Veterinary Medicine·A J BranscumW O Johnson
Jun 11, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mariana NanniNorberto Fondevila
Oct 18, 2006·BMC Veterinary Research·Barend M deC BronsvoortKenton L Morgan
Mar 29, 2008·Briefings in Bioinformatics·Kazutaka Katoh, Hiroyuki Toh
Nov 29, 2008·BMC Bioinformatics·Stephen E Hamby, Jonathan D Hirst
Dec 17, 2008·Protein Engineering, Design & Selection : PEDS·Michael J Sweredoski, Pierre Baldi
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David J PatonBryan Charleston
Aug 4, 2011·Protein Expression and Purification·Ladawan SariyaPorntippa Lekcharoensuk
Sep 6, 2011·Virology Journal·Li-na MaYong-sheng Liu
Apr 16, 2013·The EMBO Journal·Catharina SteentoftHenrik Clausen
Mar 13, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yiyang DongTakehiko Kitamori
May 28, 2014·Australian Veterinary Journal·A CollingJ R Crowther

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