PMID: 9531549May 16, 1998Paper

Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis

The Journal of Cell Biology
Beverly Wendland, S D Emr

Abstract

A genetic screen for factors required for endocytosis in the budding yeast Saccharomyces cerevisiae previously identified PAN1. Pan1p is a homologue of the mammalian protein eps15, which has been implicated in endocytosis by virtue of its association with the plasma membrane clathrin adaptor complex AP-2. Pan1p contains two eps15 homology (EH) domains, a protein-protein interaction motif also present in other proteins that function in membrane trafficking. To address the role of Pan1p and EH domains in endocytosis, a yeast two-hybrid screen was performed using the EH domain-containing region of Pan1p. This screen identified yAP180A, one of two yeast homologues of a class of clathrin assembly proteins (AP180) that exhibit in vitro clathrin cage assembly activity. In vitro binding studies using GST fusion proteins and yeast extracts defined distinct binding sites on yAP180A for Pan1p and clathrin. yAP180 proteins and Pan1p, like actin, localize to peripheral patches along the plasma membrane. Mammalian synaptojanin, a phosphatidylinositol polyphosphate-5-phosphatase, also has been implicated in endocytosis recently, and three synaptojanin-like genes have been identified in yeast. We observed genetic interactions between the yeast...Continue Reading

References

Jan 30, 1992·Nature·J E Rothman, L Orci
Nov 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D G PerryS Puszkin
Jan 1, 1990·Annual Review of Cell Biology·B M Pearse, M S Robinson
Oct 1, 1990·The Journal of Cell Biology·L A SilveiraR Schekman
Nov 29, 1985·Science·G S Payne, R Schekman
Apr 1, 1986·The Journal of Cell Biology·J H Keen, M M Black
Aug 9, 1988·Biochemistry·K Prasad, R E Lippoldt
Apr 1, 1988·Journal of Cellular Biochemistry·S LemmonE W Jones
Mar 1, 1984·The Journal of Cell Biology·J V Kilmartin, A E Adams
Jan 1, 1984·The Journal of Cell Biology·S C Mueller, D Branton
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·W T WongP P Di Fiore
Feb 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K L Carraway, C A Carraway
Jul 1, 1994·Trends in Biochemical Sciences·M G MyersM F White
Apr 1, 1994·The Journal of Cell Biology·J MulhollandD Botstein
Feb 11, 1994·Science·M ChalfieD C Prasher
Jul 1, 1993·The Journal of Cell Biology·N G DavisG F Sprague
Jan 25, 1996·Nature·P S McPhersonP De Camilli
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·C DavidP de Camilli
Mar 15, 1996·Science·R Schekman, L Orci
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·M H DreylingS K Bohlander
May 17, 1996·The Journal of Biological Chemistry·A BenmerahN Cerf-Bensussan
Jun 1, 1996·The Journal of Cell Biology·K TakeiP De Camilli
Aug 1, 1996·The Journal of Cell Biology·A F Roth, N G Davis

❮ Previous
Next ❯

Citations

Feb 5, 2005·FEMS Yeast Research·Peter H NguyenAles Vancura
Jun 30, 2000·Current Opinion in Cell Biology·D J Owen, J P Luzio
Aug 28, 1998·Current Opinion in Cell Biology·B WendlandH Riezman
Feb 18, 1999·Current Biology : CB·B J Mayer
May 20, 1999·Current Biology : CB·H T McMahon
Apr 27, 2000·Trends in Biochemical Sciences·G OdorizziS D Emr
Jan 1, 2003·Seminars in Cancer Biology·Keren Shtiegman, Yosef Yarden
Dec 4, 2001·Current Opinion in Microbiology·B L Goode, A A Rodal
Nov 30, 2002·Current Opinion in Microbiology·Daniel SchottAnthony Bretscher
Jul 23, 2011·Journal of Proteome Research·Lei ZhangMarcia M Miller
Dec 21, 2007·Leukemia·D Caudell, P D Aplan
Aug 19, 2000·The Biochemical Journal·W E HughesP J Parker
Aug 13, 2011·The Journal of Biological Chemistry·Sapan BorahRupinder Kaur
Apr 1, 2008·The Journal of Biological Chemistry·Jing-He HuangYing-Hua Chen
Feb 21, 2002·Molecular Biology of the Cell·Christopher J StefanScott D Emr
Nov 17, 1999·Molecular Biology of the Cell·B G YeungG S Payne
Mar 9, 1999·Molecular Biology of the Cell·J MulhollandD Botstein
Mar 11, 2000·Molecular Biology of the Cell·A MayerA Haas
Nov 6, 2001·Molecular Biology of the Cell·H A WatsonB Wendland
Jun 16, 2001·Molecular Biology of the Cell·G CostagutaG S Payne
Oct 20, 2006·Molecular Biology of the Cell·Irene Stavrou, Theresa J O'Halloran
Nov 24, 2006·Molecular Biology of the Cell·Ke LiuTodd R Graham
Nov 6, 2007·Molecular Biology of the Cell·Mingji Jin, Mingjie Cai
Sep 28, 2007·Molecular Biology of the Cell·Guisheng ZengMingjie Cai
May 2, 2008·Molecular Biology of the Cell·Lymarie Maldonado-BáezBeverly Wendland
Dec 23, 2011·Molecular Biology of the Cell·Ryohei SuzukiJiro Toshima
Nov 21, 1998·The EMBO Journal·S PaoluziG Cesareni
Feb 27, 1999·Protein Science : a Publication of the Protein Society·B K KayS D Emr
Dec 18, 2003·Science's STKE : Signal Transduction Knowledge Environment·Simona PoloPier Paolo Di Fiore

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Next ❯

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