Pancreatic acinar cells: acetylcholine-evoked electrical uncoupling and its ionic dependency

The Journal of Physiology
N Iwatsuki, O H Petersen

Abstract

1. Isolated segments of mouse or rat pancreas were placed in a Perspex bath through which physiological saline solutions were circulated. Intracellular recordings from surface acini were made by inserting up to three separate micro-electrodes into neighbouring cells within the same acinus. Through one of these electrodes current pulses could be injected. Electrical coupling between the cells could be continuously assessed. The acinus under investigation was stimulated by iontophoresis of ACh from an extracellular micropipette.2. During resting conditions all cells within an acinus were fully electrically coupled. A 5-10 sec pulse of a relatively large iontophoretic ACh ejecting current, causing initially the well characterized surface cell membrane depolarization and resistance reduction, was followed by a marked electrical uncoupling of neighbouring cells. In some cases one cell was clearly being isolated equally from the two other cells impaled, in other cases the cell into which current pulses were injected was becoming more isolated from one of the neighbouring cells than from the other one.3. The ACh-evoked electrical uncoupling was completely reversible and could be repeatedly observed in the same cell.4. The electrical u...Continue Reading

Citations

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