Pancreatic acinar cells: acetylcholine-evoked electrical uncoupling and its ionic dependency

The Journal of Physiology
N Iwatsuki, O H Petersen


1. Isolated segments of mouse or rat pancreas were placed in a Perspex bath through which physiological saline solutions were circulated. Intracellular recordings from surface acini were made by inserting up to three separate micro-electrodes into neighbouring cells within the same acinus. Through one of these electrodes current pulses could be injected. Electrical coupling between the cells could be continuously assessed. The acinus under investigation was stimulated by iontophoresis of ACh from an extracellular micropipette.2. During resting conditions all cells within an acinus were fully electrically coupled. A 5-10 sec pulse of a relatively large iontophoretic ACh ejecting current, causing initially the well characterized surface cell membrane depolarization and resistance reduction, was followed by a marked electrical uncoupling of neighbouring cells. In some cases one cell was clearly being isolated equally from the two other cells impaled, in other cases the cell into which current pulses were injected was becoming more isolated from one of the neighbouring cells than from the other one.3. The ACh-evoked electrical uncoupling was completely reversible and could be repeatedly observed in the same cell.4. The electrical u...Continue Reading


Jan 1, 1986·Progress in Biophysics and Molecular Biology·F Ramón, A Rivera
Sep 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·T L DyakonovaW Winlow
Jan 1, 1995·Histochemistry and Cell Biology·C HironoY Kanno
Jun 12, 1998·The Journal of Cell Biology·M ChansonPaolo Meda
Jun 1, 1994·The European Journal of Neuroscience·R AzouzY Yaari
Apr 17, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Elia Martha Pérez-ArmendarizGregorio Pérez-Palacios
Oct 1, 1988·European Journal of Clinical Investigation·R Bruzzone, Paolo Meda
Dec 15, 1989·The Journal of Comparative Neurology·E Townes-Anderson, B A Vogt
Aug 1, 1985·Pflügers Archiv : European journal of physiology·F EusebiM Stefanini
Sep 6, 1978·Pflügers Archiv : European journal of physiology·M L RobertsO H Petersen
Sep 1, 1983·Pflügers Archiv : European journal of physiology·Y Maruyama, O H Petersen
Dec 1, 1981·Pflügers Archiv : European journal of physiology·O H PetersenI Findlay
Jul 1, 1979·Pflügers Archiv : European journal of physiology·N Iwatsuki, O H Petersen
Sep 2, 1985·Nature·J Neyton, A Trautmann
Apr 28, 1978·Annals of the New York Academy of Sciences·O H Peterson, N Iwatsuki
Jun 1, 1996·Molecular Neurobiology·B Rörig, B Sutor
Dec 29, 1998·The Journal of Biological Chemistry·M ChansonH J Jongsma
Jan 17, 2020·International Journal of Molecular Sciences·Camillo Peracchia
Oct 16, 2015·Pancreas·Valentina CigliolaPaolo Meda
Jul 17, 2020·International Journal of Molecular Sciences·Camillo Peracchia

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