Pancreatic acinar cells: the effect of carbon dioxide, ammonium chloride and acetylcholine on intercellular communication

The Journal of Physiology
N Iwatsuki, O H Petersen


1. Segments of mouse pancreatic or exorbital lacrimal gland were superfused with saline solutions. Under visual control two micro-electrodes were inserted into neighbouring cells within the same acinus or into neighbouring acini. Cell to cell electrical coupling was assessed by injecting rectangular current pulses through one electrode and measuring the electrotonic potential change in the same cell (V(1)) and in the neighbouring cell (V(2)). Acetylcholine (ACh) was added locally to impaled acini by micro-ionophoresis from an extracellular micropipette.2. Exposure of the tissues to a Krebs solution equilibrated with 100% CO(2) caused a rapid increase in the size of electrotonic potential changes in the current injection cell and disappearance of the electrotonic potential changes in a neighbouring acinus or cell. This electrical uncoupling of previously coupled cells was rapidly reversible upon return to a solution equilibrated with 95% O(2) and 5% CO(2).3. Reduction of electrical intercellular coupling was also obtained using smaller CO(2) concentrations (50, 20 or 10%). In these cases the effects developed more slowly and were less dramatic. Reducing the extracellular HCO(3) concentration enhanced the uncoupling effect of 10 ...Continue Reading


Aug 5, 1987·Neuroscience Letters·M O'BeirneB A MacVicar
Jan 1, 1985·Critical Reviews in Toxicology·R Loch-Caruso, J E Trosko
Dec 1, 1981·Pflügers Archiv : European journal of physiology·O H PetersenI Findlay
Jul 1, 1979·Pflügers Archiv : European journal of physiology·N Iwatsuki, O H Petersen
Jan 1, 1992·Cell Biology and Toxicology·I V Budunova, L A Mittelman
Jun 28, 2002·Physiological Reviews·Michael C Ashby, Alexei V Tepikin
May 9, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anamika M ReedFred S Gorelick

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Mouse Pancreatic Disorder
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