PMID: 815543Jan 1, 1976

Pancreatic acinar cells: the role of calcium in stimulus-secretion coupling

The Journal of Physiology
Ole H Petersen, N Ueda


1. Segments of mouse or rat pancreas were placed in a flow cell through which physiological salt solutions of varying composition were pumped at a constant rate. Intracellular recordings of membrane potential, resistance and electrical time constant were made from the acini using fine glass micro-electrodes. In some experiments two micro-electrodes were inserted into two acinar cells within the same acinus to assess directly cell to cell coupling. The concentration of amylase in the effluent was measured continuously. 2. Electrical coupling between two acinar cells was observed when the tips of the two micro-electrodes were less than 50 mum from each other. The coupling ratio was close to 1. Acetylcholine (ACh) always evoked depolarization of exactly the same amplitude in two coupled cells and reduced the amplitude of current-pulse induced membrane potential changes in both cell simultaneously. 3. Stimulation with ACh caused an immediate increase in amylase output. Replacement of superfusion fluid Na by Tris or Cl by sulphate abolished ACh-evoked increase in amylase release, but the subsequent reintroduction of Na or Cl caused an increase in amylase release of a magnitude similar to what was normally observed following stimulat...Continue Reading


May 1, 1980·Pflügers Archiv : European journal of physiology·J W PutneyC M van de Walle
Feb 11, 2014·Cell Calcium·Ole H Petersen
Dec 31, 1979·The Journal of Membrane Biology·R K Smith, C R House
Apr 12, 1979·The Journal of Membrane Biology·I Schulz, K Heil
Nov 1, 1979·Histochemistry·D HopwoodG Nicholson
Oct 1, 1979·Digestive Diseases and Sciences·M L SteerT Manabe
Jul 1, 1977·The Histochemical Journal·T Katsuyama, S S Spicer
Jan 1, 1989·General Pharmacology·F Hertz, A Cloarec
Jun 12, 1998·The Journal of Cell Biology·M ChansonP Meda
Jul 31, 2013·The Journal of Physiology·Julia V GerasimenkoOle H Petersen
Mar 11, 2016·Cell Calcium·Oleksiy GryshchenkoOle H Petersen
May 1, 1985·The Journal of Surgical Research·K R SirinekT M O'Dorisio
Dec 1, 1986·Acta Physiologica Scandinavica·G B SagulinG M Roomans
Jul 1, 1979·Clinics in Endocrinology and Metabolism·D Powell, P Skrabanek
Sep 14, 2007·Annual Review of Physiology·Ole H Petersen, Alexei V Tepikin
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia V GerasimenkoOle H Petersen
Feb 17, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Ole H Petersen
Aug 1, 1981·Pflügers Archiv : European journal of physiology·S T Green, Ole H Petersen
Aug 1, 1985·The Anatomical Record·M BjerknesS Erlandsen
Jul 19, 1977·Pflügers Archiv : European journal of physiology·Ole H PetersenR A Hall
Mar 9, 1978·Nature·C A Prompt, P M Quinton
Jul 1, 1980·Pflügers Archiv : European journal of physiology·R Laugier, Ole H Petersen
Jan 1, 1984·Pflügers Archiv : European journal of physiology·G T Pearson, Ole H Petersen
Sep 6, 1978·Pflügers Archiv : European journal of physiology·M L RobertsOle H Petersen
Aug 29, 1977·Pflügers Archiv : European journal of physiology·N Ueda, Ole H Petersen
Jan 1, 1983·Pflügers Archiv : European journal of physiology·Ole H Petersen, Y Maruyama
Dec 1, 1981·Pflügers Archiv : European journal of physiology·Ole H PetersenI Findlay
Nov 25, 1977·Pflügers Archiv : European journal of physiology·H BauduinJ F Grenier
Sep 1, 1982·Journal of Cellular Physiology·C N Sinback, H G Coon
Apr 28, 1978·Annals of the New York Academy of Sciences·O H Peterson, N Iwatsuki
May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Aug 1, 1981·European Journal of Clinical Investigation·M C Noel-JorandH Sarles
Jul 12, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christopher M LewarchikSohail Z Husain
Jan 31, 2020·The Journal of Clinical Investigation·Sandip M SwainRodger A Liddle
Jan 1, 1977·Pflügers Archiv : European journal of physiology·Ole H PetersenT A Gray
Mar 1, 1983·Acta Pharmacologica Et Toxicologica·A LydénN G Lindquist

Related Concepts

Acetylcholine Sulfate (1: 1)
Chloride Ion Level
Egtazic Acid Sodium Salt
Electric Conductivity
Resting Potentials

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.