PMID: 815543Jan 1, 1976

Pancreatic acinar cells: the role of calcium in stimulus-secretion coupling

The Journal of Physiology
Ole H Petersen, N Ueda

Abstract

1. Segments of mouse or rat pancreas were placed in a flow cell through which physiological salt solutions of varying composition were pumped at a constant rate. Intracellular recordings of membrane potential, resistance and electrical time constant were made from the acini using fine glass micro-electrodes. In some experiments two micro-electrodes were inserted into two acinar cells within the same acinus to assess directly cell to cell coupling. The concentration of amylase in the effluent was measured continuously. 2. Electrical coupling between two acinar cells was observed when the tips of the two micro-electrodes were less than 50 mum from each other. The coupling ratio was close to 1. Acetylcholine (ACh) always evoked depolarization of exactly the same amplitude in two coupled cells and reduced the amplitude of current-pulse induced membrane potential changes in both cell simultaneously. 3. Stimulation with ACh caused an immediate increase in amylase output. Replacement of superfusion fluid Na by Tris or Cl by sulphate abolished ACh-evoked increase in amylase release, but the subsequent reintroduction of Na or Cl caused an increase in amylase release of a magnitude similar to what was normally observed following stimulat...Continue Reading

Citations

May 1, 1980·Pflügers Archiv : European journal of physiology·J W PutneyC M van de Walle
Feb 11, 2014·Cell Calcium·Ole H Petersen
Dec 31, 1979·The Journal of Membrane Biology·R K Smith, C R House
Apr 12, 1979·The Journal of Membrane Biology·I Schulz, K Heil
Nov 1, 1979·Histochemistry·D HopwoodG Nicholson
Oct 1, 1979·Digestive Diseases and Sciences·M L SteerT Manabe
Jul 1, 1977·The Histochemical Journal·T Katsuyama, S S Spicer
Jan 1, 1989·General Pharmacology·F Hertz, A Cloarec
Jun 12, 1998·The Journal of Cell Biology·M ChansonP Meda
Jul 31, 2013·The Journal of Physiology·Julia V GerasimenkoOle H Petersen
Mar 11, 2016·Cell Calcium·Oleksiy GryshchenkoOle H Petersen
May 1, 1985·The Journal of Surgical Research·K R SirinekT M O'Dorisio
Dec 1, 1986·Acta Physiologica Scandinavica·G B SagulinG M Roomans
Jul 1, 1979·Clinics in Endocrinology and Metabolism·D Powell, P Skrabanek
Sep 14, 2007·Annual Review of Physiology·Ole H Petersen, Alexei V Tepikin
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia V GerasimenkoOle H Petersen
Feb 17, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Ole H Petersen
Aug 1, 1981·Pflügers Archiv : European journal of physiology·S T Green, Ole H Petersen
Aug 1, 1985·The Anatomical Record·M BjerknesS Erlandsen
Jul 19, 1977·Pflügers Archiv : European journal of physiology·Ole H PetersenR A Hall
Mar 9, 1978·Nature·C A Prompt, P M Quinton
Jul 1, 1980·Pflügers Archiv : European journal of physiology·R Laugier, Ole H Petersen
Jan 1, 1984·Pflügers Archiv : European journal of physiology·G T Pearson, Ole H Petersen
Sep 6, 1978·Pflügers Archiv : European journal of physiology·M L RobertsOle H Petersen
Aug 29, 1977·Pflügers Archiv : European journal of physiology·N Ueda, Ole H Petersen
Jan 1, 1983·Pflügers Archiv : European journal of physiology·Ole H Petersen, Y Maruyama
Dec 1, 1981·Pflügers Archiv : European journal of physiology·Ole H PetersenI Findlay
Nov 25, 1977·Pflügers Archiv : European journal of physiology·H BauduinJ F Grenier
Sep 1, 1982·Journal of Cellular Physiology·C N Sinback, H G Coon
Apr 28, 1978·Annals of the New York Academy of Sciences·O H Peterson, N Iwatsuki
May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Aug 1, 1981·European Journal of Clinical Investigation·M C Noel-JorandH Sarles
Jul 12, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christopher M LewarchikSohail Z Husain
Jan 31, 2020·The Journal of Clinical Investigation·Sandip M SwainRodger A Liddle
Jan 1, 1977·Pflügers Archiv : European journal of physiology·Ole H PetersenT A Gray
Mar 1, 1983·Acta Pharmacologica Et Toxicologica·A LydénN G Lindquist

Related Concepts

Acetylcholine Sulfate (1: 1)
Amylases
Calcium
Chloride Ion Level
Egtazic Acid Sodium Salt
Electric Conductivity
Magnesium
Manganese
Resting Potentials
Pancreas

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