Pancreatic differentiation of induced pluripotent stem cells in activin A-grafted gelatin-poly(lactide-co-glycolide) nanoparticle scaffolds with induction of LY294002 and retinoic acid

Materials Science & Engineering. C, Materials for Biological Applications
Yung-Chih KuoRajendiran Rajesh

Abstract

The differentiation of induced pluripotent stem cells (iPSCs) in biomaterial scaffolds is an emerging area for biomedical applications. This study proposes, for the first time, the production of pancreatic cells from iPSCs in gelatin-poly(lactide-co-glycolide) nanoparticle (PLGA NP) scaffolds. The porosity and swelling ratio of the scaffolds decreased with increases in gelatin and PLGA NP concentrations. The adhesion efficiency of iPSCs in gelatin-PLGA NP scaffolds was found to be higher at 6.7% (w/w) PLGA NP. A 3-step induction of iPSCs was used to differentiate into pancreatic islet cells using activin A, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002), and retinoic acid (RA). The ability of iPSCs to differentiate into pancreatic islet cells in a scaffold was demonstrated by immunofluorescence staining and flow-cytometry analysis. The results indicate that the concentration of activin A, LY294002, and RA plays a decisive role in the differentiation of iPSCs into pancreatic cells. Activin A and LY294002 induce the iPSCs into endoderm and RA induces endoderm into islet cells. A maximum insulin secretion by glucose stimulation was obtained with a higher concentration (2μM) of RA. The use of activin A-grafted gelatin-...Continue Reading

Citations

Feb 6, 2019·Lab on a Chip·Tingting TaoJianhua Qin
Apr 22, 2020·Biotechnology and Bioengineering·Nooshin AminiSusan T Sharfstein
Jan 11, 2019·Stem Cells International·Taylor B Bertucci, Guohao Dai
Aug 23, 2020·International Journal of Molecular Sciences·Hui HuangSha Jin
May 16, 2018·Materials·Ilaria ArmentanoLuigi Torre
Dec 25, 2019·Bioactive Materials·Yibo XuXiaoping Bao
Mar 7, 2020·Cell Stem Cell·Arun SharmaClive N Svendsen
Mar 7, 2021·International Journal of Molecular Sciences·Farzane Sivandzade, Luca Cucullo
Feb 25, 2019·Advanced Drug Delivery Reviews·Caroline VissersHongjun Song

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