Oct 1, 1989

Pancreatic duct obstruction in rabbits causes digestive zymogen and lysosomal enzyme colocalization

The Journal of Clinical Investigation
Ashok SalujaM L Steer


The pancreatic duct of anesthetized rabbits was cannulated and, in some animals, flow of pancreatic exocrine secretions was blocked by raising the cannula to a vertical position. Blockage for 3-7 h caused a rapid and significant rise in serum amylase activity and an increase in amylase activity within the pancreas. The concentration of lysosomal enzymes in the pancreas was not altered but they became redistributed among subcellular fractions and, as a result, an increased amount was recovered in the 1,000-g, 15-min pellet, which was enriched in zymogen granules. Immunofluorescence studies indicated that lysosomal enzymes become localized within organelles which, in size and distribution, resemble zymogen granules. They also contain digestive enzyme zymogens. Blockage of pancreatic secretions also caused lysosomal enzyme-containing organelles to become more fragile and subject to in vitro rupture. These changes noted after short-term pancreatic duct obstruction are remarkably similar to those previously noted to occur during the early stages of diet and secretagogue-induced experimental pancreatitis, observations that have suggested that colocalization of digestive enzyme zymogens and lysosomal hydrolases might result in intrace...Continue Reading

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Mentioned in this Paper

Immunofluorescence Assay
Body Parts - Cannula
Specimen Type - Cannula
External Secretion
Pellet Formation
Acute Pancreatitis
Pancreatic Secretion Function
Benign Tumor of Pancreas

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