Pancreatic duct obstruction in rabbits causes digestive zymogen and lysosomal enzyme colocalization.

The Journal of Clinical Investigation
A SalujaM L Steer

Abstract

The pancreatic duct of anesthetized rabbits was cannulated and, in some animals, flow of pancreatic exocrine secretions was blocked by raising the cannula to a vertical position. Blockage for 3-7 h caused a rapid and significant rise in serum amylase activity and an increase in amylase activity within the pancreas. The concentration of lysosomal enzymes in the pancreas was not altered but they became redistributed among subcellular fractions and, as a result, an increased amount was recovered in the 1,000-g, 15-min pellet, which was enriched in zymogen granules. Immunofluorescence studies indicated that lysosomal enzymes become localized within organelles which, in size and distribution, resemble zymogen granules. They also contain digestive enzyme zymogens. Blockage of pancreatic secretions also caused lysosomal enzyme-containing organelles to become more fragile and subject to in vitro rupture. These changes noted after short-term pancreatic duct obstruction are remarkably similar to those previously noted to occur during the early stages of diet and secretagogue-induced experimental pancreatitis, observations that have suggested that colocalization of digestive enzyme zymogens and lysosomal hydrolases might result in intrace...Continue Reading

References

Mar 11, 1977·Virchows Archiv. A, Pathological Anatomy and Histology·M Lampel, H F Kern
Dec 1, 1988·The Journal of Cell Biology·S HashimotoJ Meldolesi
Jan 1, 1986·The Journal of Clinical Investigation·S Kornfeld
Jan 15, 1987·The New England Journal of Medicine·M L Steer, J Meldolesi
Feb 28, 1974·The New England Journal of Medicine·J M Acosta, C L Ledesma
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Oct 1, 1984·Digestive Diseases and Sciences·M L SteerC Figarella

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Citations

Mar 5, 2004·Gastrointestinal Endoscopy·Enzo Masci, Alberto Mariani
Dec 10, 1999·Journal of the American College of Surgeons·S RätyI Nordback
Mar 17, 1999·Journal of Clinical Gastroenterology·J T Frakes
Jun 30, 2000·Journal of Clinical Gastroenterology·G H Sakorafas, A G Tsiotou
Jan 2, 2007·Pancreas·Yuk Cheung Chan, Po Sing Leung
Aug 17, 2011·Current Opinion in Gastroenterology·Raghuwansh P Sah, Ashok Saluja
Aug 14, 2012·Current Opinion in Gastroenterology·Raghuwansh P SahAshok K Saluja
Oct 16, 2008·Journal of Gastroenterology and Hepatology·Alain VonlaufenMinoti V Apte
Oct 25, 2006·Annual Review of Physiology·Ashok K SalujaVikas Dudeja
Sep 11, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anna S Gukovskaya, Ilya Gukovsky
Jan 1, 1992·The Journal of Clinical Investigation·T UedaY Saitoh
Mar 27, 2009·World Journal of Gastroenterology : WJG·Guo-Jun WangSi-Qin Ding
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M BhatiaM L Steer
Nov 1, 1991·Digestive Diseases and Sciences·K N Rao, D H Van Thiel
Mar 18, 2015·Redox Biology·Salvador PérezJuan Sastre
Oct 16, 2007·American Journal of Surgery·David K Meyerholz, Isaac Samuel
Apr 25, 2006·Endocrinology and Metabolism Clinics of North America·Rodger A Liddle
Nov 25, 2006·Gastroenterology·Roland M Schmid, David C Whitcomb
Mar 26, 1998·Baillière's Clinical Gastroenterology·M G RaratyJ P Neoptolemos
Nov 6, 2004·Gastroenterology Clinics of North America·Rodger A Liddle
Feb 19, 2008·Free Radical Biology & Medicine·Javier PeredaJuan Sastre
Jan 1, 1992·Scandinavian Journal of Gastroenterology. Supplement·S D LeachI M Modlin
Oct 24, 2003·The American Journal of Gastroenterology·Enzo MasciUNKNOWN Gabexate in Digestive Endoscopy-Italian Group II

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