PMID: 6397027Oct 1, 1984Paper

Pancreatic islet metabolism and redox state during stimulation of insulin secretion with glucose and fructose

Acta diabetologica latina
K CapitoP Thams

Abstract

The mechanism of potentiation of insulin secretion by fructose was investigated. Twenty mM fructose + 3 mM glucose stimulated insulin secretion in a biphasic manner similar to what is found during stimulation with 20 mM glucose, whereas 20 mM fructose alone did not affect secretion. Fructose utilization was measured as formation of tritiated water from 5-3H-fructose. At 27.8 mM fructose the utilization rate was 258 pmol/2 h/10 islets, which is less than the utilization rate of 2.8 mM glucose. 20 mM glucose increased the islet NADH/NAD+ and NADPH/NADP+ redox ratios as well as islet concentration of ATP and PEP. 20 mM fructose + 3 mM glucose did not affect the concentration of ATP and PEP or the NADH/NAD+ redox ratio. The NADPH/NADP+ ratio was significantly decreased (60%) after 2.5 min incubation with .20 mM fructose + 3 mM glucose. It is concluded that fructose potentiation of insulin secretion is not primarily dependent on fructose metabolism and that any conceivable effect on plasma membrane ion fluxes as caused by a reduction of plasma membrane disulfides, may be caused by mechanisms other than a mere increase in the pyridine nucleotide substrates for the transhydrogenation process.

References

Apr 1, 1979·Metabolism: Clinical and Experimental·W J MalaisseJ C Hutton
Jun 1, 1970·The Biochemical Journal·S J AshcroftP J Randle
Apr 1, 1952·Biochimica Et Biophysica Acta·H G HERS

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Citations

Oct 6, 1999·Molecular and Cellular Endocrinology·A P SalgadoR Ramasamy
Feb 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·George A KyriazisBjörn Tyrberg
May 3, 2012·American Journal of Physiology. Endocrinology and Metabolism·Joshua P GrayEmma A Heart
Sep 11, 1989·FEBS Letters·W J MalaisseE Van Schaftingen
Jul 5, 1990·European Journal of Biochemistry·W J MalaisseE Van Schaftingen
Feb 15, 1988·Archives of Biochemistry and Biophysics·A Sener, W J Malaisse
Mar 17, 2016·Biochimica Et Biophysica Acta·Thierry Brun, Pierre Maechler
Jun 25, 2013·Molecular and Cellular Endocrinology·Pierre Maechler
Nov 22, 2012·Best Practice & Research. Clinical Endocrinology & Metabolism·Pierre Maechler
Jun 23, 2011·Theoretical Biology & Medical Modelling·Peter Buchwald
Jun 20, 2006·Biochimica Et Biophysica Acta·Marie-Hélène GiroixAbdullah Sener
Mar 27, 2019·American Journal of Physiology. Endocrinology and Metabolism·Clarissa BartleyPierre Maechler
Feb 21, 2019·Anais Da Academia Brasileira De Ciências·Carolina A MirandaKleber E Campos

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