Pandemic 2009 H1N1 influenza virus causes diffuse alveolar damage in cynomolgus macaques

Veterinary Pathology
S HerfstT Kuiken

Abstract

The pathogenesis of lower respiratory tract disease from the pandemic 2009 H1N1 (H1N1v) influenza A virus is poorly understood. Therefore, either H1N1v virus or a seasonal human H1N1 influenza A virus was inoculated into cynomolgus macaques as a nonhuman primate model of influenza pneumonia, and virological, pathological, and microarray analyses were performed. Macaques in the H1N1v group had virus-associated diffuse alveolar damage involving both type I and type II alveolar epithelial cells and affecting an average of 16% of the lung area. In comparison, macaques in the seasonal H1N1 group had milder pulmonary lesions. H1N1v virus tended to be reisolated from more locations in the respiratory tract and at higher titers than seasonal H1N1 virus. In contrast, differential expression of messenger RNA transcripts between H1N1v and seasonal H1N1 groups did not show significant differences. The most upregulated genes in H1N1v lung samples with lesions belonged to the innate immune response and proinflammatory pathways and correlated with histopathological results. Our results demonstrate that the H1N1v virus infects alveolar epithelial cells and causes diffuse alveolar damage in a nonhuman primate model. Its higher pathogenicity com...Continue Reading

References

Jun 20, 2001·Journal of Virology·G F RimmelzwaanA D Osterhaus
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Isabelle PhamChristine Clerici
Feb 26, 2004·Nature Medicine·Bart L HaagmansAlbert D M E Osterhaus
Jul 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Donald R OlsonStephen S Morse
Mar 25, 2006·Science·Debby van RielThijs Kuiken
Sep 4, 2007·Advances in Virus Research·Jamie L FornekMichael G Katze
Nov 28, 2007·Annual Review of Pathology·Jeffery K Taubenberger, David M Morens
Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Carole R BaskinMichael G Katze
May 9, 2009·The New England Journal of Medicine·Fatimah S DawoodTimothy M Uyeki

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Citations

May 15, 2013·Archives of Virology·Kun ZhangXianzhu Xia
Nov 19, 2010·Journal of Virology·David SafronetzHeinz Feldmann
Jun 8, 2011·The Journal of Veterinary Medical Science·Ryosuke KobayashiKinji Shirota
Mar 4, 2014·Journal of Comparative Pathology·J M A van den BrandT Kuiken
Oct 10, 2014·Clinical and Vaccine Immunology : CVI·Jason A SkinnerGerard Zurawski
Dec 17, 2016·Emerging Infectious Diseases·Miranda de GraafMarion P G Koopmans
Apr 17, 2021·Infection and Drug Resistance·Liang ChenXiqian Xing

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Datasets Mentioned

BETA
EPI178291

Methods Mentioned

BETA
light microscopy
Chip

Software Mentioned

Affymetrix
limma
Ingenuity Pathways Knowledge Base
Gene Chip Operating Software
Spotfire DecisionSite

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