Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids

Scientific Reports
Yuki YokoiTokiyoshi Ayabe

Abstract

Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota. However, Paneth cell secretory responses remain debatable and the mechanisms that regulate the secretion are not well understood. Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota. Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an ex vivo system for studying Paneth cell secretion in real-time. The results show that Paneth cells released granules immediately when the apical surfaces of enteroid epithelial cells were exposed to LPS or live bacteria by microinjection. However, Paneth cells did not respond to LPS delivered in culture media to enteroid exterior basolateral surface, although they responded to basolateral carbamyl choline. In addition, Paneth cells replenished...Continue Reading

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Citations

May 1, 2020·Frontiers in Immunology·Jan Wehkamp, Eduard F Stange
Sep 16, 2020·Anatomical Science International·Siriporn ChamniansawatNarongrit Thongon
Sep 23, 2020·Nature Materials·Jonathan A BrassardMatthias P Lutolf
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Nov 6, 2020·Current Opinion in Gastroenterology·Rishu Dheer, Vincent B Young
Dec 10, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anne S Ten HoveWouter J de Jonge
Nov 16, 2020·Microbes and Infection·Shawn GoyalStephen E Girardin
Nov 7, 2020·Frontiers in Immunology·Kiminori NakamuraTokiyoshi Ayabe
Apr 26, 2021·Cellular and Molecular Gastroenterology and Hepatology·Xiao HanBruce A Vallance
Jun 22, 2021·Cellular and Molecular Gastroenterology and Hepatology·Paul CrayChristopher M Dekaney
Aug 8, 2021·International Journal of Molecular Sciences·Akihiko InabaKen Iwatsuki
Jan 15, 2022·Proceedings of the National Academy of Sciences of the United States of America·Mariko KamiokaHiroshi Kiyono
Jan 16, 2022·Cell Biology International·Laylla Barreto E BarretoPatrícia Gama

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Methods Mentioned

BETA
confocal laser microscopy
confocal microscopy
Fluorescence
gel-filtration

Software Mentioned

GraphPad
GraphPad Prism
NIS
Elements

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