Panicovirus accumulation is governed by two membrane-associated proteins with a newly identified conserved motif that contributes to pathogenicity

Virology Journal
Jeffrey S BattenK B Scholthof

Abstract

Panicum mosaic virus (PMV) has a positive-sense, single-stranded RNA genome that serves as the mRNA for two 5'-proximal genes, p48 and p112. The p112 open reading frame (ORF) has a GDD-motif, a feature of virus RNA-dependent RNA polymerases. Replication assays in protoplasts showed that p48 and p112 are sufficient for replication of PMV and its satellite virus (SPMV). Differential centrifugation of extracts from PMV-infected plants showed that the p48 and p112 proteins are membrane-associated. The same fractions exhibited RNA polymerase activity in vitro on viral RNA templates, suggesting that p48 and p112 represent the viral replication proteins. Moreover, we identified a domain spanning amino acids 306 to 405 on the p48 and p112 PMV ORFs that is common to the Tombusviridae. Alanine scanning mutagenesis of the conserved domain (CD) revealed that several substitutions were lethal or severely debilitated PMV accumulation. Other substitutions did not affect RNA accumulation, yet they caused variable phenotypes suggestive of plant-dependent effects on systemic invasion and symptom induction. The mutants that were most debilitating to PMV replication were hydrophobic amino acids that we hypothesize are important for membrane locali...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C W CollmerS H Howell
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jan 1, 1994·Advances in Virus Research·M RussoG P Martelli
Aug 1, 1996·The Journal of General Virology·J BurgyanM Russo
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jan 8, 1999·Virology·L Rubino, M Russo
May 3, 2001·Journal of Virology·W Qiu, K B Scholthof
Jul 17, 2001·Cleveland Clinic Journal of Medicine·B H Cohen, D R Gold
Sep 20, 2002·Journal of Virology·Frédérique Weber-LotfiLuisa Rubino
Jul 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael SchwartzPaul Ahlquist
Oct 1, 1999·Plant Disease·Over Cabrera, Karen-Beth G Scholthof

❮ Previous
Next ❯

Citations

Nov 28, 2013·PloS One·Liang Fang, Robert H A Coutts
Nov 6, 2014·Molecular Plant-microbe Interactions : MPMI·Cristina Gómez-AixMaría Amelia Sánchez Pina
May 2, 2006·FEBS Letters·Jeffrey S BattenKaren-Beth G Scholthof
Jul 28, 2016·Archives of Virology·Shu Hui KohStephen J Wylie
Jun 1, 2016·Virus Research·Kay Scheets

❮ Previous
Next ❯

Datasets Mentioned

BETA
U55002
X14736
Y13463
M29671
M22445
L16015
X02986
X83964

Methods Mentioned

BETA
X-ray
electron microscopy
transgenic
electrophoresis

Software Mentioned

PSI
BLAST
BLASTP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.