Panitumumab-IRDye800CW for Fluorescence-Guided Surgical Resection of Colorectal Cancer

The Journal of Surgical Research
John C MarstonJason M Warram

Abstract

Fluorescence-guided surgery (FGS) is a rapidly advancing field that may improve outcomes in several cancer types. Although screening has decreased colorectal cancer (CRC) mortality, it remains a common and often fatal malignancy. In this study, we sought to identify an optical imaging agent for the application of FGS technology to CRC. We compared a panitumumab-IRDye800CW conjugate to an IgG-IRDye800CW isotype control. Mice were implanted with one of three CRC cell lines (LS174T, Colo205, and SW948) and imaged with open- and closed-filed fluorescence imaging systems. Fluorescent contrast was quantified by calculating the ratio between tumor and background fluorescence. After 10 d, the mice were sacrificed, and their tumors stained for microscopic imaging. Panitumumab-IRDye800CW produced significantly greater (P < 0.05) fluorescent contrast in all three cell lines. Average tumor to background ratio was 6.00 versus 2.60 for LS174T, 5.78 versus 2.52 for Colo205, and 4.31 versus 1.70 for SW948. A 1-mg tumor fragment produced significantly greater fluorescent contrast in the Colo205 and SW948 cell lines in the panitumumab-IRDye800CW group. Western blotting for epidermal growth factor receptor (EGFR) and a semiquantitative analysis o...Continue Reading

Citations

May 30, 2019·Frontiers in Pharmacology·Pieterjan Debie, Sophie Hernot
Dec 15, 2020·Surgical Oncology·Hannah M HollandsworthMichael Bouvet
Jan 26, 2021·Small·Abraham S MosesOleh Taratula
Sep 4, 2020·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Jacqueline PeiEben L Rosenthal
May 19, 2021·Journal of Biomedical Optics·Feredun AzariSunil Singhal
Sep 9, 2021·Nature Reviews. Clinical Oncology·J Sven D MieogAlexander L Vahrmeijer

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