Pankiller effect of prolonged exposure to menadione on glioma cells: potentiation by vitamin C.

Investigational New Drugs
Marina F VitaJuan Sebastian Yakisich

Abstract

Menadione (Vitamin K3) has anti-tumoral effects against a wide range of cancer cells. Its potential toxicity to normal cells and narrow therapeutic range limit its use as single agent but in combination with radiation or other anti-neoplastic agents can be of therapeutic use. In this paper, we first evaluated the early (within 3 h) effect of menadione on ongoing DNA replication. In normal rat cerebral cortex mini-units menadione showed an age dependent anti-proliferative effect. In tissue mini-units prepared from newborn rats, menadione inhibited ongoing DNA replication with an IC (50) of approximately 10 μM but 50 μM had no effect on mini-units from prepared adult rat tissue. The effect of short (72 h) and prolonged exposure (1-2 weeks) to menadione alone in the DBTRG.05MG human glioma cells line and in combination with vitamin C was studied. After short period of exposure data show that menadione alone or in combination with vitamin C provided similar concentration-response curves (and IC(50) values). Prolonged exposure to these drugs was evaluated by their ability to kill 100% of glioma cells and prevent regrowth when cells are re-incubated in drug-free media. In this long-term assay, menadione:vitamin C at a ratio 1:100 sho...Continue Reading

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Citations

May 10, 2011·Investigational New Drugs·Zahid M DelwarJuan Sebastian Yakisich
Dec 23, 2015·Journal of Experimental & Clinical Cancer Research : CR·Tiantian HeMeng-Er Huang
Sep 22, 2017·Scandinavian Journal of Clinical and Laboratory Investigation·Sofia DahlbergUlf Schött
May 1, 2021·Antioxidants·Daniela-Saveta PopaMarius Emil Rusu

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