Pankinetoplast DNA structure in a primitive bodonid flagellate, Cryptobia helicis

The EMBO Journal
J LukescaronO Benada

Abstract

The mitochondrial DNA (mtDNA) of a primitive kinetoplastid flagellate Cryptobia helicis is composed of 4.2 kb minicircles and 43 kb maxicircles. 85% and 6% of the minicircles are in the form of supercoiled (SC) and relaxed (OC) monomers, respectively. The remaining minicircles (9%) constitute catenated oligomers composed of both the SC and OC molecules. Minicircles contain bent helix and sequences homologous to the minicircle conserved sequence blocks. Maxicircles encode typical mitochondrial genes and are not catenated. The mtDNA, which we describe with the term 'pankinetoplast DNA', is spread throughout the mitochondrial lumen, where it is associated with multiple electron-lucent loci. There are approximately 8400 minicircles per pankinetoplast-mitochondrion, with the pan-kDNA representing approximately 36% of the total cellular DNA. Based on the similarity of the C.helicis minicircles to plasmids, we present a theory on the formation of the kDNA network.

References

May 1, 1991·Trends in Genetics : TIG·P Borst
Feb 1, 1981·Molecular and Biochemical Parasitology·C CastroM Castañeda
Jan 13, 1995·Cell·J ChenN R Cozzarelli
Aug 1, 1994·The Journal of Cell Biology·D R Robinson, K Gull
Mar 24, 1994·Nature·D A MaslovL Simpson
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A P FernandesS M Beverley
Nov 1, 1995·Molecular & General Genetics : MGG·A J Griffiths, X Yang
Jan 1, 1995·Annual Review of Microbiology·T A Shapiro, P T Englund
Jul 1, 1995·Molecular and Biochemical Parasitology·M JirkůJ Lukes
Jun 3, 1996·Biochimica Et Biophysica Acta·G J Arts, R Benne
Feb 7, 1997·Science·J D Palmer
May 1, 1997·Journal of Molecular Evolution·J LukesD A Maslov

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Citations

May 6, 2011·Current Genetics·Pavel FlegontovJulius Lukeš
Oct 14, 2003·Experimental Parasitology·Alena ZíkováJulius Lukes
Mar 25, 2000·International Journal for Parasitology·J E Feagin
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·S Brewster, D C Barker
Apr 11, 2001·Molecular and Biochemical Parasitology·P StolbaJ Lukes
Jun 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Julius LukesPatrick J Keeling
Oct 12, 2010·Nucleic Acids Research·Cestmir VlcekGertraud Burger
Nov 26, 2002·Molecular Biology and Evolution·Alastair G B SimpsonAndrew J Roger
Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Adrian C BarbrookSarah J Tarr
Jul 23, 1999·Annals of the New York Academy of Sciences·L Simpson, D A Maslov
Nov 29, 2002·Eukaryotic Cell·Julius LukesPaul T Englund
Jun 11, 2005·Eukaryotic Cell·William MarandeGertraud Burger
Feb 13, 2001·Experimental Parasitology·J Lukes, J Votýpka
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·L SimpsonD A Maslov
Jul 15, 1999·Experimental Parasitology·V YurchenkoJ Lukes
Jun 4, 2014·Molecular and Biochemical Parasitology·Julius LukešVyacheslav Yurchenko
Oct 7, 2018·IUBMB Life·Julius LukešJohn M Archibald
Sep 25, 2004·International Journal of Systematic and Evolutionary Microbiology·David MoreiraKeith Vickerman
Oct 12, 2018·IUBMB Life·Gertraud Burger, Matus Valach
Mar 16, 2021·Open Biology·Alexei Y KostygovJulius Lukeš

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