Pannexin 1 Channels Contribute to IL-1β Expression via NLRP3/Caspase-1 Inflammasome in Aspergillus Fumigatus Keratitis

Current Eye Research
Xuejiao YangJie Zhang

Abstract

Purpose: Pannexin 1 channels are deemed to play important roles in inflammation. However, there is limited information regarding their roles in fungal infection diseases, especially fungal keratitis. This study aimed to investigate the role of pannexin 1 channels in Aspergillus fumigatus (A. fumigatus) keratitis. Materials and Methods: Mouse models or immortalized human corneal epithelial cells (HCECs) were infected with or without A. fumigatus for given time. The expression of pannexin 1 channels was tested by qPCR, western blot and immunofluorescence staining. Mice of A. fumigatus keratitis were pretreated with carbenoxolone (CBX) or 2'(3')-O-(4-Benzoylbenzoyl) adenosine-5'-triphosphate (BzATP) to block or activate the opening of pannexin 1 channels respectively. The clinical score was recorded. Cornea tissues were examined for the downstream signals of pannexin 1 channels, including NLRP3, Caspase-1 and IL-1β, and myeloperoxidase (MPO) by PCR and ELISA. Data were analyzed with commercial data analysis software and a P < 0.05 was considered to be statistically significant. Results: Upon A. fumigatus infection, pannexin 1 expression increased at both the mRNA and the protein levels in mice corneas (P< 0.05, n = 3). Immunofluor...Continue Reading

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Citations

Apr 30, 2020·Investigative Ophthalmology & Visual Science·Lu ZhanGuiqiu Zhao
Feb 6, 2020·International Journal of Molecular Sciences·Rayne R LimShyam S Chaurasia
Mar 10, 2020·Oxidative Medicine and Cellular Longevity·Zheng WangLi Li
Feb 20, 2021·Frontiers in Immunology·Jin Kyung SeokJoo Young Lee
Jul 3, 2021·International Journal of Molecular Sciences·Erin M O'ShaughnessyPatricia E Martin
Nov 30, 2021·IScience·Ross LuuEliseo Eugenin
Jan 23, 2022·Environmental Science and Pollution Research International·Xiang ZengXia Huo

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