Pannexin-2 is expressed in the human colon with extensive localization in the enteric nervous system

Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society
E F DiezmosL Liu

Abstract

Pannexin-2 (Panx2) is a member of the novel group of membrane spanning protein channels present in the central nervous system. Limited studies have examined Panx2 in the intestine, where it may have important physiological roles. The present study characterized Panx2 expression and localization in the human colon in health and disease states. Immunofluorescence determined Panx2 localization and co-localization, and quantitative real-time PCR and Western blot determined gene and protein expression in ulcerative colitis (UC), Crohn's disease (CD), and control human colon. Panx2 was widely expressed in myenteric and submucosal ganglia, particularly in the cytoplasm of neurons. Panx2 was also expressed on smooth muscle of the muscularis and blood vessels, some non-lymphoid leukocytes, mast cells, and mucosal epithelial cells. Co-localization of Panx2 occurred with β-tubulin, neuronal nitric oxide synthase, substance P, vesicular acetylcholine transporter, and calcitonin gene-related peptide, indicating widespread Panx2 expression in extrinsic and intrinsic neurons. Molecular studies revealed a 3.4-fold higher level of Panx2 mRNA in ascending compared to sigmoid muscularis (p < 0.05), despite similar protein levels. Similarly, UC mu...Continue Reading

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Citations

Jun 21, 2015·Cellular and Molecular Life Sciences : CMLS·Lilian I Plotkin, Joseph P Stains
Jul 26, 2016·Frontiers in Neuroscience·Erica F DiezmosLu Liu
Mar 16, 2017·Journal of Cellular Physiology·Jin XuLanfang Li
Jun 1, 2017·Biochimica Et Biophysica Acta. Biomembranes·Silvana ValdebenitoEliseo A Eugenin

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