Pannexin1-mediated ATP release provides signal transmission between Neuro2A cells.

Neurochemical Research
R Iglesias, David C Spray

Abstract

Pannexin1 (Panx1), a protein related to the gap junction proteins of invertebrates, forms nonjunctional channels that open upon depolarization and in response to mechanical stretch and purinergic receptor stimulation. Importantly, ATP can be released through Panx1 channels, providing a possible role for these channels in non-vesicular signal transmission. In this study we expressed exogenous human and mouse Panx1 in the gap junction deficient Neuro2A neuroblastoma cell line and explored the contribution of Panx1 channels to cell-cell communication as sites of ATP release. Electrophysiological (patch clamp) recordings from Panx1 transfected Neuro2A cells revealed membrane conductance that increased beyond 0 mV when applying voltage ramps from -60 to +100 mV; threshold was correlated with extracellular K+, so that at 10 mM K+, channels began to open at -30 mV. Evaluation of cell-cell communication using dual whole cell recordings from cell pairs revealed that activation of Panx1 current in one cell of the pair induced an inward current in the second cell after a latency of 10-20 s. This paracrine response was amplified by an ATPase inhibitor (ARL67156, 100 μM) and was blocked by the ATP-degrading enzyme apyrase (6.7 U/ml), by the...Continue Reading

References

Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jorge E ContrerasJuan C Sáez
Jan 16, 2002·The Journal of Biological Chemistry·Charles E StoutAndrew C Charles
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Roberto BruzzoneHannah Monyer
Aug 12, 2004·FEBS Letters·Li BaoGerhard Dahl
Sep 7, 2005·Brain Research. Molecular Brain Research·Angelika VogtHannah Monyer
Mar 28, 2006·Physiology·Michael T BarbeRoberto Bruzzone
May 13, 2006·Science·Roger J ThompsonBrian A MacVicar
Sep 26, 2006·The Cerebellum·Arundhati RayRolf Dermietzel
Sep 29, 2006·Glia·Eliana Scemes, Christian Giaume
Sep 29, 2006·Glia·David C SprayBruce R Ransom
Jan 20, 2007·The EMBO Journal·Roman A RomanovStanislav S Kolesnikov
Jan 24, 2007·FEBS Letters·Silviu LocoveiGerhard Dahl
Feb 20, 2007·Cancer Research·Charles P K LaiChristian C Naus
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yi-Jen HuangStephen D Roper
May 2, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jian KangMaiken Nedergaard
Jul 2, 2008·Nature Reviews. Drug Discovery·Geoffrey Burnstock
Jul 4, 2008·American Journal of Physiology. Cell Physiology·R IglesiasE Scemes
Jul 19, 2008·Neuron Glia Biology·Eliana ScemesDavid C Spray
Aug 16, 2008·Molecular Neurobiology·Carlos Matute
Oct 15, 2008·Pflügers Archiv : European journal of physiology·Eliana ScemesPaolo Meda
Oct 24, 2008·American Journal of Physiology. Cell Physiology·Feng Qiu, Gerhard Dahl
Nov 22, 2008·The Journal of Pharmacology and Experimental Therapeutics·Weihong MaAnnmarie Surprenant
May 7, 2009·The Journal of Biological Chemistry·William R SilvermanGerhard Dahl
May 29, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rodolfo IglesiasEliana Scemes
Jul 9, 2011·Pharmacological Reviews·Claudio CoddouStanko S Stojilkovic

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Citations

Jul 16, 2014·Frontiers in Physiology·Kurt A SchalperMauricio P Oyarzo
Mar 29, 2014·American Journal of Physiology. Cell Physiology·Albert LeeVickery Trinkaus-Randall
May 13, 2017·The Biochemical Journal·Andrew K J Boyce, Leigh Anne Swayne
Feb 14, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Calum WilsonJohn G McCarron
Dec 1, 2015·Cellular & Molecular Biology Letters·Kamila PuchałowiczDariusz Chlubek
Nov 1, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Pamela Cabahug-ZuckermanMitchell B Schaffler
Sep 29, 2020·Frontiers in Cellular Neuroscience·Kseniia KorolevaGuzel Sitdikova
Jan 5, 2021·Molecular and Cellular Biochemistry·Eijaz Ahmed Bhat, Nasreena Sajjad
Jan 17, 2013·Journal of Cell Science·Gonzalo JorqueraMariana Casas

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