Panobinostat, a histone deacetylase inhibitor, rescues the angiogenic potential of endothelial colony-forming cells in moyamoya disease

Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery
Anshika JangraSeung-Ki Kim

Abstract

Moyamoya disease (MMD) is one of the most common causes of pediatric stroke. We found defective angiogenic function and downregulation of retinaldehyde dehydrogenase 2 (RALDH2) in MMD endothelial colony-forming cells (ECFCs). Downregulation of RALDH2 mRNA was caused by decreased binding of acetyl-histone H3 (Ac-H3) to the RALDH2 promoter. In this study, we evaluated the feasibility of using a histone deacetylase (HDAC) inhibitor, panobinostat, to upregulate RALDH2 expression and restore the angiogenic potential of MMD ECFCs. ECFCs from healthy normal controls and patients with MMD were isolated and characterized. After panobinostat treatment, western blot, tube formation, and chromatin immunoprecipitation (ChIP) assays were conducted in vitro. A matrigel plug assay was performed in vivo. Panobinostat increased the levels of Ac-H3 and Ac-H4 in both normal and MMD ECFCs but was much more effective in MMD ECFCs. Increased expression of RALDH2 by panobinostat was observed only in MMD ECFCs. Panobinostat increased the tube formation of both normal and MMD ECFCs in vitro and in vivo, but the effect was greater with MMD ECFCs. We demonstrated that panobinostat increases the angiogenic ability of MMD ECFCs by regulating RALDH2 acetylat...Continue Reading

References

Jan 1, 1983·Stroke; a Journal of Cerebral Circulation·M YamashitaK Tanaka
Oct 22, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M H Kuo, C D Allis
Jan 5, 2001·Experimental Cell Research·S G Gray, T J Ekström
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David Z QianRoberto Pili
Sep 7, 2006·Nature Reviews. Drug Discovery·Jessica E BoldenRicky W Johnstone
Oct 20, 2007·Circulation Research·Denis MottetVincent Castronovo
Dec 20, 2008·Stroke; a Journal of Cerebral Circulation·Neysan RafatPeter Vajkoczy
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Rosa KarlićMartin Vingron
May 29, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Claire BouvardDominique Helley
Oct 29, 2010·Trends in Pharmacological Sciences·André FischerLi-Huei Tsai
Apr 25, 2012·Journal of Visualized Experiments : JoVE·Nutan PrasainMervin C Yoder
Nov 15, 2012·Sub-cellular Biochemistry·Sharmila A Bapat
Jun 12, 2013·Molecular Systems Biology·Preeti JoshiIleana M Cristea
Dec 30, 2014·The FEBS Journal·Sylvain FraineauMarjorie Brand
Jun 21, 2015·Journal of Community Hospital Internal Medicine Perspectives·Imtiaz IsmailRohit Arora
Oct 18, 2016·Chinese Medical Journal·Yong-Gang MaJi-Zong Zhao
Jul 4, 2017·International Journal of Molecular Sciences·Tomas EckschlagerJan Hrabeta

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Citations

Aug 17, 2020·International Journal of Molecular Sciences·Francesca TinelliLaura Gatti
Oct 11, 2020·International Journal of Molecular Sciences·Pawan FarisFrancesco Moccia

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