Pantethine Alters Lipid Composition and Cholesterol Content of Membrane Rafts, With Down-Regulation of CXCL12-Induced T Cell Migration

Journal of Cellular Physiology
Manuel van Gijsel-BonnelloBouchra Gharib

Abstract

Pantethine, a natural low-molecular-weight thiol, shows a broad activity in a large range of essential cellular pathways. It has been long known as a hypolipidemic and hypocholesterolemic agent. We have recently shown that it exerts a neuroprotective action in mouse models of cerebral malaria and Parkinson's disease through multiple mechanisms. In the present study, we looked at its effects on membrane lipid rafts that serve as platforms for molecules engaged in cell activity, therefore providing a target against inappropriate cell response leading to a chronic inflammation. We found that pantethine-treated cells showed a significant change in raft fatty acid composition and cholesterol content, with ultimate downregulation of cell adhesion, CXCL12-driven chemotaxis, and transendothelial migration of various T cell types, including human Jurkat cell line and circulating effector T cells. The mechanisms involved include the alteration of the following: (i) CXCL12 binding to its target cells; (ii) membrane dynamics of CXCR4 and CXCR7, the two CXCL12 receptors; and (iii) cell redox status, a crucial determinant in the regulation of the chemokine system. In addition, we considered the linker for activation of T cells molecule to sh...Continue Reading

References

Nov 25, 1988·Biochimica Et Biophysica Acta·G CighettiM Galli Kienle
Jan 1, 1980·Journal of Immunological Methods·W FalkE J Leonard
Sep 1, 1982·Atherosclerosis·S RanganathanJ A Harmony
Aug 4, 1999·Immunology Today·V HorejsíH Stockinger
Oct 20, 1999·The Journal of Cell Biology·P W JanesA I Magee
Jan 8, 2000·The Journal of Biological Chemistry·C O RockS Jackowski
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·A SaccaniA Sica
Apr 20, 2000·The Journal of Biological Chemistry·D A Brown, E London
Mar 29, 2001·Nature Immunology·C Gerard, B J Rollins
Mar 29, 2001·Nature Immunology·B Moser, P Loetscher
Apr 11, 2001·Advances in Immunology·T Harder
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·C Gómez-MóutonC Martínez-A
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·R L Wange
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dzung H Nguyen, Dennis Taub
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·François Van LaethemOberdan Leo
Nov 5, 2003·Experimental Cell Research·Dzung H Nguyen, Dennis D Taub
Feb 26, 2004·The Journal of Cell Biology·Concepción Gómez-MoutónSantos Mañes
Mar 3, 2004·BioTechniques·Renaldo C Drisdel, William N Green
Jun 9, 2004·Trends in Cell Biology·Santos Mañes, Carlos Martínez-A
Feb 25, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yi LeLeslie E Silberstein
Aug 19, 2005·The Journal of Biological Chemistry·Karl BalabanianFrançoise Bachelerie
Apr 28, 2006·Nature Reviews. Immunology·John GreenwoodScott S Zamvil
Jul 22, 2006·The EMBO Journal·Pierre-François LenneDidier Marguet
Nov 2, 2006·Science's STKE : Signal Transduction Knowledge Environment·Marilyn D Resh
Sep 14, 2007·European Journal of Immunology·Pascal DuchesneauThomas K Waddell
Jan 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Marie-France PenetBouchra Gharib

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Citations

Sep 26, 2015·Digestive Diseases and Sciences·Mariana Verdelho MachadoAnna Mae Diehl
Jan 18, 2018·Biological Chemistry·Lena CollenburgElita Avota
Nov 10, 2015·Biochemical Society Transactions·Kirsty E Waddington, Elizabeth C Jury
Jul 4, 2019·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Kevin BarangerPhilippe Benech
Dec 3, 2016·Frontiers in Oncology·Marie-France PenetZaver M Bhujwalla

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