PMID: 5844079Nov 5, 1965

Papain membrane on a collodion matrix: preparation and enzymic behavior

Science
R GoldmanE Katchalski

Abstract

A stable papain membrane has been prepared on a collodion matrix by absorbing papain in a collodion membrane and then cross-linking the papain with bisdiazobenzidine 3,3'-disulfonic acid. The pH-dependence of the activity of the enzyme membrane on the low-molecular-weight substrate, benzoylarginine ethyl ester, was found to differ from that of crystalline papain; the activity was low in the neutral pH range where the native enzyme has its optimum and high at alkaline pH. This anomalous behavior is due to a lowering of the local pH within the membrane as a result of the release of acid by the enzymic hydrolysis of the ester substrate.

Citations

May 1, 1970·Biotechnology and Bioengineering·H H Weetall, G Baum
Apr 1, 1974·Bulletin of Mathematical Biology·B Bunow
Oct 8, 1968·Biochimica Et Biophysica Acta·S N PenningtonC O Knowles
Jul 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J H KennedyP Wilding
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·K D Munkres, D O Woodward
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·H I Silman, A Karlin
Dec 1, 1979·CRC Critical Reviews in Food Science and Nutrition·A Kilara, K M Shahani
Aug 25, 1971·European Journal of Biochemistry·T C Laurent
Jul 7, 1978·Biochimica Et Biophysica Acta·T T Ngo, K J Laidler
Apr 22, 1970·Biochimica Et Biophysica Acta·H H Weetall, L S Hersh
Nov 4, 1969·Biochimica Et Biophysica Acta·R AxénA Winter
Jan 1, 1969·Biochimica Et Biophysica Acta·H H Weetall, L S Hersh
Dec 1, 1968·Journal of Biomedical Materials Research·S N PenningtonS K Chattopadhyay
Feb 1, 1968·Biochemistry·R GoldmanE Katchalski
Jan 5, 1971·Biochemistry·R GoldmanE Katchalski
May 31, 1974·Nature·A NaparstekD Thomas
Oct 19, 1973·Nature·S R CaplanN J Zabusky
Apr 1, 1966·Analytical Chemistry·G G Guilbault
Jan 1, 1986·Histochemistry·M van der Ploeg, W A Duijndam

Related Concepts

Amides
Arginine hydrochloride
Celloidin
Tromasin
Sulfonic Acids

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