PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation

The Journal of Cell Biology
Olivia LuuRudolf Winklbauer

Abstract

Cleft-like boundaries represent a type of cell sorting boundary characterized by the presence of a physical gap between tissues. We studied the cleft-like ectoderm-mesoderm boundary in Xenopus laevis and zebrafish gastrulae. We identified the transcription factor Snail1 as being essential for tissue separation, showed that its expression in the mesoderm depends on noncanonical Wnt signaling, and demonstrated that it enables paraxial protocadherin (PAPC) to promote tissue separation through two novel functions. First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation. Second, PAPC controls formation of a distinct type of adhesive contact between mesoderm and ectoderm cells that shows properties of a cleft-like boundary at the single-cell level. It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps. These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.

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Citations

Oct 6, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ashley E E Bruce
Mar 18, 2015·The Journal of Cell Biology·David G Wilkinson
Aug 11, 2015·Seminars in Cell & Developmental Biology·Hervé Turlier, Jean-Léon Maître
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Jul 1, 2020·Seminars in Cell & Developmental Biology·François Fagotto
Jul 28, 2020·Seminars in Cell & Developmental Biology·François Fagotto
Sep 22, 2021·Proceedings of the National Academy of Sciences of the United States of America·Debanjan BaruaRudolf Winklbauer

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Datasets Mentioned

BETA
BC056857.1

Methods Mentioned

BETA
antisense oligonucleotides
fluorescence overlay
GTPases
light microscopy
scraping
fluorescence microscopy

Software Mentioned

LSM
ADSA
LasAF
Volocity
Openlab
AxioVision

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