Paper-Based Antibody Detection Devices Using Bioluminescent BRET-Switching Sensor Proteins.

Angewandte Chemie
Keisuke TendaDaniel Citterio

Abstract

This work reports on fully integrated "sample-in-signal-out" microfluidic paper-based analytical devices (μPADs) relying on bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation. The devices use BRET-based antibody sensing proteins integrated into vertically assembled layers of functionalized paper, and their design enables sample volume-independent and fully reagent-free operation, including on-device blood plasma separation. User operation is limited to the application of a single drop (20-30 μL) of sample (serum, whole blood) and the acquisition of a photograph 20 min after sample introduction, with no requirement for precise pipetting, liquid handling, or analytical equipment except for a camera. Simultaneous detection of three different antibodies (anti-HIV1, anti-HA, and anti-DEN1) in whole blood was achieved. Given its simplicity, this type of device is ideally suited for user-friendly point-of-care testing in low-resource environments.

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Citations

Apr 3, 2020·Micromachines·Muqsit Pirzada, Zeynep Altintas
Nov 24, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Meng-Meng PanLi Xu
Nov 27, 2020·Biochemical Society Transactions·Lieuwe BiewengaMaarten Merkx
Dec 29, 2020·Proceedings. Mathematical, Physical, and Engineering Sciences·José Alvim BerkenbrockSven Achenbach
Mar 19, 2021·Chemical Society Reviews·Aisha J Syed, James C Anderson
Mar 20, 2021·Analytica Chimica Acta·Yoshiki Soda, Eric Bakker
Apr 22, 2021·Lab on a Chip·Wen-Chin LeeLung-Ming Fu
Jun 15, 2021·Chemical Reviews·Eka NovianaCharles S Henry
Jul 31, 2021·Biomicrofluidics·Hao YuanChien-Fu Chen
Aug 14, 2021·Biosensors & Bioelectronics·Jiantao PingQiongzheng Hu
Dec 19, 2019·ACS Sensors·Alejandra Alba-PatiñoRoberto de la Rica
Aug 18, 2021·Journal, Genetic Engineering & Biotechnology·Rola NadeemMahmoud M Bahgat
Mar 17, 2020·Analytical Chemistry·Kento MaejimaDaniel Citterio
Nov 27, 2019·Analytical Chemistry·Ling XiaGongke Li
Oct 15, 2020·ACS Sensors·Hope Adamson, Lars J C Jeuken

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Methods Mentioned

BETA
BRET

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