Paper-based DPPH Assay for Antioxidant Activity Analysis

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Kitima SirivibulkovitYupaporn Sameenoi

Abstract

We report on a paper-based 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) assay for a simple, inexpensive, low reagent and sample consumption and high throughput analysis of antioxidant activity. The paper-based device was fabricated using a lamination method to create a 5-mm in diameter circular test zone that was embedded with a DPPH reagent. The analysis was carried out in one-step by dropping an antioxidant/sample onto the test zone. After reduction by the antioxidant, the DPPH radicals become stable DPPH molecules, resulting in a change in color from deep violet to pale yellow. The violet color intensity of DPPH was inversely proportional to the antioxidant activity of the samples, and was measured using imaging software. A high precision and a low limit of detection were found in the analysis of six standard antioxidants including gallic acid, trolox, ascorbic acid, caffeic acid, vanilliic acid and quercetin. The device was then validated against the traditional spectrophotometric DPPH assay by analyzing the antioxidant activity of 7 tea samples. The results showed no significant difference for gallic acid equivalent for all 7 samples obtained from the two methods at the 95% confidence level, indicating that the dev...Continue Reading

References

Feb 6, 2002·The Analyst·Michael AntolovichKevin Robards
Mar 20, 2002·Phytochemical Analysis : PCA·Irina I KolevaLyuba N Evstatieva
Aug 22, 2002·Journal of Agricultural and Food Chemistry·Anne C KurilichBarbara P Klein
Jan 5, 2006·Journal of Agricultural and Food Chemistry·Anne T HukkanenHarri I Kokko
Sep 28, 2006·Journal of Agricultural and Food Chemistry·Zhihong ChengLiangli Yu
Aug 14, 2008·Analytical Chemistry·Koji AbeDaniel Citterio
Jan 15, 2007·Talanta·D VillañoM C García-Parrilla
Mar 15, 2007·Talanta·Ivana Murković Steinberg, Stjepan Milardović
Mar 27, 2010·Analytical Chemistry·Emanuel CarrilhoGeorge M Whitesides
Jan 28, 2009·ACS Applied Materials & Interfaces·Erin M FentonScott S Sibbett
May 27, 2010·Journal of Agricultural and Food Chemistry·Alfreda Wei, Takayuki Shibamoto
Sep 15, 2010·Free Radical Research·Agnieszka AugustyniakNeven Zarković
Nov 26, 2010·Analytical Chemistry·Juuso OlkkonenTomi Erho
Mar 31, 2012·Brazilian Dental Journal·Eugenio José GarciaRosa Helena Miranda Grande
Dec 12, 2012·Environmental Science & Technology·Yupaporn SameenoiJohn Volckens
Dec 18, 2013·Biosensors & Bioelectronics·Jie HuFeng Xu
Apr 18, 2014·Journal of Agricultural and Food Chemistry·J Xie, K M Schaich
Nov 7, 2014·Analytical Chemistry·David M CateCharles S Henry
Sep 22, 2015·Journal of Agricultural and Food Chemistry·Mario C Foti
Oct 17, 2015·Food Chemistry·Claudia Grajeda-IglesiasMaria Cruz Figueroa-Espinoza
Feb 26, 2016·Journal of Diabetes Science and Technology·Busadee PratumvinitPanutsaya Tientadakul
Dec 19, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Remya KomeriJayabalan Muthu
Jan 22, 2017·Analytica Chimica Acta·Ricardo A G de OliveiraRonaldo Censi Faria
Jan 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Piyakorn WorramongkonaWijitar Dungchai
Jan 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hitoshi Asano, Yukihide Shiraishi
Jan 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Purim JarujamrusSuparb Tamuang
Jan 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Thirada PiyananYupaporn Sameenoi

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Citations

May 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Trina H GarcíaLuca Rastrelli
Apr 25, 2020·Journal of Food Science and Technology·Bambang KuswandiIndah Yulia Ningsih
May 1, 2021·Materials·Monika ParchetaWłodzimierz Lewandowski
May 29, 2021·Journal of Biochemical and Molecular Toxicology·Luiz Roberto Carraro JuniorCristiani Folharini Bortolatto
Jun 5, 2021·Natural Product Research·Yang-Cheng LiuChang-Lan Chen
Jan 13, 2022·Natural Product Research·Arnold Alexeevich ShamilovEkaterina Robertovna Garsiya

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