Paper-based enzymatic platform coupled to screen printed graphene-modified electrode for the fast neonatal screening of phenylketonuria

Clinica Chimica Acta; International Journal of Clinical Chemistry
Cristian M MoreiraGermán A Messina

Abstract

The PKU is an inborn error of amino acid metabolism, in which phenylalanine (Phe) accumulated in the blood causing alterations at the central nervous system. We report a novel paper-based enzymatic platform coupled to screen printed graphene-modified electrode for the neonatal screening of phenylketonuria (PKU0. The paper-based analytical device coupled to electrochemical detection (EPAD) is based on the use of paper microzones modified with phenylalanine dehydrogenase enzyme (PheDH). The modified PADs were placed on the surface of an electrode modified with electrochemically reduced graphene (ERGO). PheDH in the presence of NAD+ catalyzes the reversible deamination of Phe to form phenylpyruvate, ammonia, and NADH. The electrochemical oxidation of NADH was monitored by differential pulse amperometry (DPA) at 0.6 V. The method was linear in the concentration range from 1 to 600 μmol/L of Phe with a LOQ of 1 μmol/L and LOD of 0.2 μmol/L. Within day precision was 5.7% across 3 levels of control samples. Between-day precision was 8.3%. The comparison with the standard Phe enzyme assay kit showed good agreement. The time required for the overall assay was <5 min. The non-sophisticated equipment required, the short assay time and the...Continue Reading

Citations

Jul 15, 2020·Biosensors·Paloma Yáñez-SedeñoJosé Manuel Pingarrón
Mar 7, 2021·Biosensors·Estefanía Costa-Rama, María Teresa Fernández-Abedul
Nov 2, 2020·International Journal of Biological Macromolecules·Baoting SunShiru Jia
Apr 22, 2021·Lab on a Chip·Wen-Chin LeeLung-Ming Fu
Jun 15, 2021·Chemical Reviews·Eka NovianaCharles S Henry

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