ParA proteins of secondary genome elements cross-talk and regulate radioresistance through genome copy number reduction in Deinococcus radiodurans

The Biochemical Journal
Ganesh Kumar MauryaHari S Misra

Abstract

Deinococcus radiodurans, an extremely radioresistant bacterium has a multipartite genome system and ploidy. Mechanisms underlying such types of bacterial genome maintenance and its role in extraordinary radioresistance are not known in this bacterium. Chromosome I (Chr I), chromosome II (Chr II) and megaplasmid (Mp) encode its own set of genome partitioning proteins. Here, we have characterized P-loop ATPases of Chr II (ParA2) and Mp (ParA3) and their roles in the maintenance of genome copies and extraordinary radioresistance. Purified ParA2 and ParA3 showed nearly similar polymerization kinetics and interaction patterns with DNA. Electron microscopic examination of purified proteins incubated with DNA showed polymerization on nicked circular dsDNA. ParA2 and ParA3 showed both homotypic and heterotypic interactions to each other, but not with ParA1 (ParA of Chr I). Similarly, ParA2 and ParA3 interacted with ParB2 and ParB3 but not with ParB1 in vivo ParB2 and ParB3 interaction with cis-elements located upstream to the corresponding parAB operon was found to be sequence-specific. Unlike single mutant of parA2 and parA3, their double mutant (ΔparA2ΔParA3) affected copy number of cognate genome elements and resistance to γ-radiati...Continue Reading

References

May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Mar 14, 2000·Current Biology : CB·J R Battista
Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew T G HoldenJulian Parkhill
Dec 23, 2004·Molecular Microbiology·Michael A Fogel, Matthew K Waldor
May 11, 2005·Molecular Microbiology·Elizabeth S EganMatthew K Waldor
Jan 18, 2006·Nature Reviews. Microbiology·Finbarr Hayes, Daniela Barillà
Jul 21, 2006·Journal of Bacteriology·Djenann Saint-DicLyn Sue Kahng
Dec 13, 2006·Genes & Development·Michael A Fogel, Matthew K Waldor
Dec 15, 2006·Molecular Microbiology·Jean-Yves BouetDavid Lane
Dec 22, 2006·PloS One·Sebastian BreuertJörg Soppa
Oct 2, 2008·Mikrobiologiia·A A Prozorov
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Simon RinggaardKenn Gerdes
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Monica P HuiEdward H Egelman
Apr 7, 2010·PLoS Computational Biology·Vaughn S CooperPhilip J Hatcher
Jun 17, 2010·Cell·Kenn GerdesFlorian Szardenings
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Conrad W ShebelutZemer Gitai
Jan 25, 2011·Journal of Bacteriology·Ryosuke KadoyaDhruba K Chattoraj
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Dea Slade, Miroslav Radman
Jul 31, 2012·Methods : a Companion to Methods in Enzymology·Aurélia Battesti, Emmanuelle Bouveret
Dec 31, 2014·Nature Methods·Jianyi YangYang Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.