PMID: 9178630Jul 1, 1997Paper

Paracellular calcium transport across Caco-2 and HT29 cell monolayers

Pflügers Archiv : European journal of physiology
A BlaisB Lacour

Abstract

Intestinal calcium absorption has been shown to include two processes, a saturable transcellular movement and a non-saturable paracellular pathway. The potential utility of cell monolayers for studying transepithelial intestinal calcium transport has already been demonstrated; however, simultaneous evaluation of the contribution of the saturable transcellular and of the non-saturable paracellular processes to the total transepithelial transport has not yet been attempted. The aim of this study was to investigate the contribution both of transcellular and paracellular transport processes to the total transepithelial calcium transport in two cell culture monolayers. Caco-2 cells and a clone derived from HT-29 cells (HT29-Cl.19A), two cell lines derived from colon adenocarcinomas which are known to be able to exhibit typical enterocytic differentiation, were used. Cell monolayers were grown on a permeable support and used after 15 days of culture when these cells express enterocytic differentiation and high transepithelial resistance. Isotopic transport rate measurements were performed in the absence of a chemical gradient. The paracellular route was evaluated using [3H]mannitol. Calcium and [3H]mannitol transport rates across cel...Continue Reading

Citations

Apr 30, 2010·European Journal of Nutrition·Houda Sabboh-JourdanMichael J Gidley
Nov 26, 2011·American Journal of Physiology. Cell Physiology·Jayshree MishraNarendra Kumar
Feb 20, 2007·International Journal of Pharmaceutics·S M MoyesK E Carr
Sep 1, 2015·Journal of Agricultural and Food Chemistry·Tao HouHui He
Nov 21, 2012·Nature Reviews. Gastroenterology & Hepatology·Michel NeunlistMalvyne Rolli-Derkinderen
Jun 22, 2019·The Journal of Physiological Sciences : JPS·Kannikar WongdeeNarattaphol Charoenphandhu
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