Paracellular pathway remodeling enhances sodium secretion by teleost fish in hypersaline environments

The Journal of Experimental Biology
Regina R F CozziWilliam S Marshall

Abstract

In vertebrate salt-secreting epithelia, Na(+) moves passively down an electrochemical gradient via a paracellular pathway. We assessed how this pathway is modified to allow Na(+) secretion in hypersaline environments. Mummichogs (Fundulus heteroclitus) acclimated to hypersaline [2× seawater (2SW), 64‰] for 30 days developed invasive projections of accessory cells with an increased area of tight junctions, detected by punctate distribution of CFTR (cystic fibrosis transmembrane conductance regulator) immunofluorescence and transmission electron miscroscopy of the opercular epithelia, which form a gill-like tissue rich in ionocytes. Distribution of CFTR was not explained by membrane raft organization, because chlorpromazine (50 μmol l(-1)) and filipin (1.5 μmol l(-1)) did not affect opercular epithelia electrophysiology. Isolated opercular epithelia bathed in SW on the mucosal side had a transepithelial potential (Vt) of +40.1±0.9 mV (N=24), sufficient for passive Na(+) secretion (Nernst equilibrium voltage≡ENa=+24.11 mV). Opercular epithelia from fish acclimated to 2SW and bathed in 2SW had higher Vt of +45.1±1.2 mV (N=24), sufficient for passive Na(+) secretion (ENa=+40.74 mV), but with diminished net driving force. Bumetanide ...Continue Reading

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Citations

Aug 5, 2015·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Phuong Bui, Scott P Kelly
May 24, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Christian K TipsmarkE Gordon Grau
Nov 19, 2017·The Journal of Experimental Biology·William S MarshallPatricia M Schulte
Mar 31, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Alex M ZimmerChris N Glover
Apr 13, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Leighann Martin, Andrew J Esbaugh

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