Paraclostridium bifermentans exacerbates pathosis in a mouse model of ulcerative colitis

PloS One
Ryo KutsunaYoshiaki Kawamura

Abstract

Although it has been recognized that intestinal bacteria play an important role in the pathology of human ulcerative colitis (UC), specific pathogenic bacteria for UC have not been identified. We investigated the influence of Paraclostridium bifermentans PAGU1678 strain on the pathology of a UC mouse model and found it increased UC pathosis scores such as loose and bloody stools, reduced diversity of fecal flora, disappearance of the crypt structure of distal colon tissue, destruction of intestinal epithelial cells, and atrophy of the colon. Furthermore, we observed an increase in COX-2, TNF-α, IL-6, IL-1, and IL-17 expression and a decrease in Foxp3 and SOCS3 expression, as inflammation-related factors and inflammatory cytokines, a decrease in the concentration of short chain fatty acids (acetic acid, propionic acid, and butyric acid) in feces, and an increase of intestinal mucosal myeloperoxidase activity. These results suggest that P. bifermentans PAGU1678 is a pathology-exacerbating factor in a mouse model of UC. This study is the first to demonstrate exacerbation of the pathological condition in a mouse model of UC by a single bacterial strain.

References

Sep 26, 1991·The New England Journal of Medicine·D K Podolsky
Oct 3, 1991·The New England Journal of Medicine·D K Podolsky
Jan 27, 1995·International Journal of Cancer. Journal International Du Cancer·A HagueC Paraskeva
Nov 1, 1994·Journal of Clinical Microbiology·D R ScanlanE Hilton
Oct 30, 2001·Digestive Diseases and Sciences·S ChoudharyA Banan
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masahide MiyamotoAnders Lindén
Oct 16, 2004·Immunity·Jay K Kolls, Anders Lindén
Feb 4, 2005·Journal of Leukocyte Biology·Seymour J Klebanoff
May 17, 2006·Anaerobe·Cynthia L Sears
Sep 18, 2008·Biochemical and Biophysical Research Communications·Reiko ItoOsam Mazda
Apr 17, 2009·World Journal of Gastroenterology : WJG·Hai-Qiang ZhangYun-Long Cui
Jun 6, 2009·The Journal of Physiology·Peter J Turnbaugh, Jeffrey I Gordon
May 1, 2002·FEMS Microbiology Ecology·Julien LoubinouxAlain E Faou
Sep 10, 2009·The Journal of Experimental Medicine·Soraya TalebZiad Mallat
Sep 2, 2010·World Journal of Gastroenterology : WJG·Sahar K Hegazy, Mohamed M El-Bedewy
Dec 21, 2011·Nature Reviews. Gastroenterology & Hepatology·Thomas J Borody, Alexander Khoruts
May 12, 2012·Journal of Gastroenterology·Akira AndohTakayuki Matsumoto
Aug 22, 2012·Nature Reviews. Gastroenterology & Hepatology·Chaysavanh ManichanhFrancisco Guarner
Oct 30, 2012·Pharmacology & Therapeutics·Tadakazu HisamatsuToshifumi Hibi
Feb 26, 2016·Journal of Microbiology and Biotechnology·Sung Wook HongMi-Ai Lee

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Methods Mentioned

BETA
GAM
dissection
electrophoresis
PCR
ELISA

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