Paracoccus pantotrophus pseudoazurin is an electron donor to cytochrome c peroxidase

Biochemistry
Sofia R PauletaG W Pettigrew

Abstract

The gene for pseudoazurin was isolated from Paracoccus pantotrophus LMD 52.44 and expressed in a heterologous system with a yield of 54.3 mg of pure protein per liter of culture. The gene and protein were shown to be identical to those from P. pantotrophus LMD 82.5. The extinction coefficient of the protein was re-evaluated and was found to be 3.00 mM(-1) cm(-1) at 590 nm. It was confirmed that the oxidized protein is in a weak monomer/dimer equilibrium that is ionic-strength-dependent. The pseudoazurin was shown to be a highly active electron donor to cytochrome c peroxidase, and activity showed an ionic strength dependence consistent with an electrostatic interaction. The pseudoazurin has a very large dipole moment, the vector of which is positioned at the putative electron-transfer site, His81, and is conserved in this position across a wide range of blue copper proteins. Binding of the peroxidase to pseudoazurin causes perturbation of a set of NMR resonances associated with residues on the His81 face, including a ring of lysine residues. These lysines are associated with acidic residues just back from the rim, the resonances of which are also affected by binding to the peroxidase. We propose that these acidic residues moder...Continue Reading

References

Oct 11, 1991·Biochimica Et Biophysica Acta·N EllfolkK Osterlund
Nov 1, 1990·The Biochemical Journal·C F GoodhewG W Pettigrew
Dec 15, 1988·The Biochemical Journal·P M HannaD R McMillin
Dec 1, 1972·Journal of Bacteriology·B J WilkinsonD C White
Dec 2, 1998·Journal of Biomolecular Structure & Dynamics·G CastroS H Northrup
Apr 12, 2001·European Journal of Biochemistry·M F OttenR J Van Spanning
Dec 3, 2003·The Journal of Biological Chemistry·Jason CrackAndrew J Thomson

❮ Previous
Next ❯

Citations

Mar 16, 2012·Biochemistry·Julian SeidelOliver Einsle
Jun 3, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Shabir NajmudinMaria J Romão
Mar 22, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Félix M FerroniCarlos D Brondino
Mar 16, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·P M Paes de SousaJ J G Moura
Mar 28, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·P M Paes de SousaM M Correia Dos Santos
Aug 23, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Isabel MouraJosé J G Moura
Jul 9, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Simone Dell'acquaSofia R Pauleta
Aug 23, 2012·Journal of Inorganic Biochemistry·Koyu FujitaDavid M Dooley
Aug 5, 2009·Journal of Inorganic Biochemistry·Rui M AlmeidaJosé J G Moura
Sep 17, 2013·Chembiochem : a European Journal of Chemical Biology·Rui M AlmeidaSofia R Pauleta
Jan 26, 2006·Journal of Inorganic Biochemistry·Graham W PettigrewSofia R Pauleta
Sep 9, 2009·Journal of Molecular Biology·Maren HoffmannOliver Einsle
Aug 16, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui M AlmeidaSofia R Pauleta
Apr 25, 2008·Methods in Enzymology·Sarah J FieldNicholas J Watmough
Apr 8, 2017·Journal of Inorganic Biochemistry·Cláudia S NóbregaSofia R Pauleta
Feb 29, 2020·Chemical Reviews·Christina FerousiKyle M Lancaster
Jan 13, 2006·Chemical Reviews·Ivano BertiniAntonio Rosato
Jun 9, 2005·Chemical Reviews·Gérard Simonneaux, Arnaud Bondon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.