Paracrine and endocrine modes of myostatin action.

Journal of Applied Physiology
Yun-Sil LeeSe-Jin Lee

Abstract

Myostatin (MSTN) is a secreted signaling molecule that normally acts to limit muscle mass. In adult animals, MSTN is made almost exclusively by skeletal muscle and circulates in the blood. A critical question is whether this circulating MSTN protein can enter the active pool to regulate muscle growth or whether all of the activity of MSTN results from locally produced protein. Here, we addressed this question in mice by using a Cdx2-Cre transgene in conjunction with a conditional Mstn-flox allele to generate mice in which Mstn was targeted in a regionally restricted manner. Specifically, we generated mosaic mice in which MSTN production was eliminated in posteriorly located muscles but not in anteriorly located muscles, resulting in mice in which circulating levels of MSTN were reduced roughly by half. Analysis of posteriorly located vs. anteriorly located muscles of these mice revealed clear differential effects indicative of an important paracrine role for MSTN in regulating muscle mass. Significant, albeit more subtle, effects consistent with an endocrine mode of MSTN action were also seen in these mice. These findings have important implications not only for the understanding of the physiological control of muscle mass but ...Continue Reading

References

Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·M LaksoH Westphal
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·A C McPherron, S J Lee
Mar 21, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L GrobetM Georges
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·N F Gonzalez-CadavidS Bhasin
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 25, 2002·Science·Teresa A ZimmersSe-Jin Lee
Jun 25, 2004·The New England Journal of Medicine·Markus SchuelkeSe-Jin Lee
Mar 23, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Astrid BreitbartJoerg Heineke
Jan 1, 2010·Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry·Se-Jin Lee
Apr 24, 2013·The International Journal of Biochemistry & Cell Biology·Ana Isabel PadrãoRita Ferreira
Dec 22, 2014·Biochemical and Biophysical Research Communications·Estibaliz CastilleroIsaac George
May 24, 2015·Cell Metabolism·Marc A EgermanDavid J Glass

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Citations

Dec 6, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yuda WeiQiurong Ding
Nov 24, 2017·Journal of Cachexia, Sarcopenia and Muscle·Srinivasan Dasarathy
May 14, 2016·Circulation Research·Buel D Rodgers
Nov 28, 2018·Human Molecular Genetics·Kimberly K LongAdriana Donovan
Mar 17, 2020·Journal of Atherosclerosis and Thrombosis·Pasquale EspositoGiacomo Garibotto
Aug 18, 2017·Physiological Reviews·Frank W BoothRyan G Toedebusch
Sep 22, 2019·International Journal of Molecular Sciences·Paula Renee ChenKichoon Lee
Oct 30, 2018·Journal of the American Heart Association·Joshua T ButcherDavid W Stepp
Apr 9, 2017·Cold Spring Harbor Perspectives in Medicine·Christoph Hoffmann, Cora Weigert
Oct 21, 2020·Experimental & Molecular Medicine·Joonho Suh, Yun-Sil Lee
Nov 22, 2020·Proceedings of the National Academy of Sciences of the United States of America·Se-Jin LeeThomas A Rando
Dec 8, 2020·American Journal of Physiology. Endocrinology and Metabolism·Masayuki KobayashiMasao Kaneki
Feb 13, 2021·International Journal of Environmental Research and Public Health·Remigiusz DominPrzemysław Guzik
Apr 1, 2021·Journal of Physiology and Biochemistry·Liang TangDean Ta
Aug 14, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yewei LiuSe-Jin Lee

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Methods Mentioned

BETA
transgenic
PCR
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

AxioVision
Image
Gen5 Data Analysis
Pro Premier

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