Paracrine effect of human adipose-derived stem cells on lymphatic endothelial cells.

Regenerative Medicine
Cristiana MarcozziRosalba Gornati

Abstract

Aim: The proposal of this study was to evaluate, in vitro, the potential paracrine effect of human adipose-derived stem cells (hASCs) to promote lymphangiogenesis in lymphatic endothelial cells isolated from rat diaphragmatic lymphatic vessels. Materials & methods: ELISA on VEGFA, VEGFC and IL6 in hASC-conditioned medium; LYVE1 immunostaining; and gene expression of PROX1, VEGFR3, VEGFC, VEGFA and IL6 were the methods used. Results: In 2D culture, hASC-conditioned medium was able to promote lymphatic endothelial cell survival, maintenance of endothelial cobblestone morphology and induction to form a vessel-like structure. Conclusion: The authors' results represent in vitro evidence of the paracrine effect of hASCs on lymphatic endothelial cells, suggesting the possible role of hASC-conditioned medium in developing new therapeutic approaches for lymphatic system-related dysfunction such as secondary lymphedema.

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Datasets Mentioned

BETA
AF106860.2
AF402786.1

Methods Mentioned

BETA
dissection
medical procedure
flow cytometry
PCR
electrophoresis
hELISA
ELISA

Software Mentioned

Leica Chantal
Beacon Designer
SigmaPlot
ImageJ

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