Paracrine effect of inflammatory cytokine-activated bone marrow mesenchymal stem cells and its role in osteoblast function

Journal of Bioscience and Bioengineering
Cheng LiHaibin Zhou

Abstract

Mesenchymal stem cells (MSCs) have a crucial function in bone regeneration. Inflammation is a well-documented component of the osteogenic microenvironment. In the present study, we investigated whether stimulation of MSCs with inflammatory cytokines promotes osteogenesis through a paracrine mediator. MSCs were pre-stimulated with the inflammatory factors IFN-γ and TNF-α. After pre-stimulation, the MSC secretion levels of IL-6, HGF, VEGF, and TGF-β were significantly elevated (p < 0.01); however, the production of IL-2, IL-4, and IL-10 was not changed (p > 0.05). MG63, an osteoblast-like cell line, was cultured in different MSC-conditioned media. After treatment with conditioned media collected from MSCs pre-treated with cytokines, the proliferation and migration of MG63 cells were significantly improved, and the expression levels of the osteoblast differentiation markers ALP, COLI, OCN and OPN were significantly increased as revealed by a quantitative PCR analysis (p < 0.05). Furthermore, an immunofluorescence staining assay showed that more MG63 cells were OPN-positive, while an Alizarin red staining indicated the increased formation of calcium nodules in the IFN-γ and TNF-α combined pretreatment group. The results indicated t...Continue Reading

References

Feb 28, 1994·Biochemical and Biophysical Research Communications·V Midy, J Plouët
Dec 16, 2003·Arthritis and Rheumatism·J Mary MurphyFrank P Barry
Sep 24, 2005·Annals of Neurology·Jacques De Keyser
Oct 21, 2005·Cytotherapy·E M HorwitzUNKNOWN International Society for Cellular Therapy
Apr 19, 2006·Journal of Cellular Biochemistry·Arnold I Caplan, James E Dennis
May 12, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·J J J P van den BeuckenJ A Jansen
May 30, 2006·Transplantation·Olle RingdénKatarina Le Blanc
Oct 3, 2006·European Journal of Immunology·Antonio UccelliVito Pistoia
May 15, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ming ZhangMarc S Penn
Sep 23, 2009·Tissue Engineering. Part a·Longmei ZhaoBasil M Hantash
Feb 8, 2011·Biochimica Et Biophysica Acta·Jürgen SchellerStefan Rose-John
Oct 24, 2012·Immunology and Cell Biology·Karen English

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Citations

Feb 24, 2016·Frontiers in Physiology·Consuelo Merino-GonzálezClaudio Aguayo
Mar 11, 2016·Drug Discoveries & Therapeutics·Chengzhi LiJinxiang Han
Aug 18, 2016·In Vitro Cellular & Developmental Biology. Animal·Guijuan FengDongmei Zhang
Jan 18, 2018·Journal of Cellular Physiology·Qingyi ZhangHengxiang Wang
May 21, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Índia O de Azevedo QueirozJoão E Gomes-Filho
Feb 17, 2018·Médecine sciences : M/S·Hugo OliveiraJean-Christophe Fricain
Jan 24, 2016·Stem Cell Research & Therapy·Florence LoiZhenyu Yao
Oct 3, 2018·Stem Cells International·Jolene PhelpsArindom Sen
Feb 8, 2019·Stem Cells International·Yi WangJie Tian
May 11, 2019·Journal of Cellular Physiology·Ruinan SunZuolin Wang
Jan 26, 2020·Journal of Clinical Medicine·David AlfaroAgustín G Zapata
Jan 26, 2017·Frontiers in Endocrinology·Marjorie E BatemanBruce A Bunnell
Jan 27, 2019·Bioengineering & Translational Medicine·Varsha V RaoKristi S Anseth
Jul 24, 2020·Cellular and Molecular Life Sciences : CMLS·Luis A CostaFrancisco J Vizoso
Oct 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xingyu ZhangShiyi Chen
Jan 29, 2021·World Journal of Stem Cells·Alberto González-GonzálezFlor M Pérez-Campo
Jul 6, 2020·Trends in Biotechnology·Leila DaneshmandiCato T Laurencin
Jul 3, 2021·International Journal of Molecular Sciences·Sarah Hani ShoushrahEdda Tobiasch
Sep 2, 2021·Journal of Tissue Engineering and Regenerative Medicine·Mengsong WuJun Liu

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