Paracrine effects of bFGF and KGF on the process of mouse blastocyst implantation

Molecular Reproduction and Development
F TaniguchiN Terakawa

Abstract

Implantation is a complex process that requires the interaction of the blastocyst, and subsequently, that of the developing embryos with the endometrium. Several growth factors and cytokines are involved in implantation, but the details of their actions as related to the regulation of blastocyst implantation remain unclear. In the present study, the RT-PCR method was used to determine the gene expression of basic fibroblast growth factor (bFGF), keratinocyte growth factor (KGF), FGF receptor 1 (FGFR1), FGF receptor 2 (FGFR2), and KGF receptor (KGFR) in mouse embryos and in the stromal and epithelial cells of the uterine endometrium. Basic FGF and KGF mRNA were expressed in the endometrial cells, but were not expressed in the embryos. The mRNAs of receptors for bFGF and KGF were expressed in the blastocysts and in the in vitro implanting embryos, suggesting that bFGF and KGF may exert paracrine effects on blastocyst implantation. In this mouse model of blastocyst implantation, it was found that transforming growth factor alpha (TGF-alpha) at the concentrations of 1 ng/ml and 10 ng/ml significantly enhanced the blastocyst attachment and trophoblast spreading and increased trophoblast surface area. Relatively high concentrations o...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T MikiS A Aaronson
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A TanakaK Matsumoto
Sep 1, 1992·Journal of Reproduction and Fertility·R J WordingerT L Jackson
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H H ReidO Bernard
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J S RubinS A Aaronson
Jul 1, 1995·Acta Obstetricia Et Gynecologica Scandinavica·S SiegfriedM Ammälä
Jan 13, 1995·Science·B FeldmanM Goldfarb
Sep 1, 1993·Molecular and Cellular Endocrinology·F PekonenE M Rutanen

❮ Previous
Next ❯

Citations

Nov 6, 2004·Journal of Assisted Reproduction and Genetics·Fuminori TaniguchiNaoki Terakawa
Mar 8, 2005·The Journal of Reproduction and Development·Koji MiyazakiHiroshi Nagashima
Nov 11, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Flavia N T CookeAlan D Ealy
Feb 21, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·L A OkumuP Lonergan
Mar 14, 2017·Reproductive Sciences·Gulcin Sahin ErsoyHugh S Taylor
May 24, 2017·Zygote : the Biology of Gametes and Early Embryos·Peter L PfefferDebra K Berg
Nov 27, 2015·Biological Reviews of the Cambridge Philosophical Society·Eline WydoogheAnn Van Soom
Mar 30, 2000·American Journal of Obstetrics and Gynecology·F TaniguchiN Terakawa
Mar 30, 2002·Nature Biotechnology·Joanne M YeakleyXiang-Dong Fu
May 8, 2008·Current Opinion in Obstetrics & Gynecology·Kevin S Richter
May 4, 2004·The Journal of Steroid Biochemistry and Molecular Biology·H WelterR Einspanier
Sep 17, 2009·Mechanisms of Development·Ahmed H K El-HashashSusan J Kimber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.