Paracrine factors from human placental multipotent mesenchymal stromal cells protect endothelium from oxidative injury via STAT3 and manganese superoxide dismutase activation

Biology of Reproduction
Shu-Hsiang LiuChie-Pein Chen

Abstract

Reactive oxygen species may cause oxidative damage in the placenta, yet some mechanisms must exist to reduce or prevent such damage. We investigated whether oxidative injury to placental endothelial cells is inhibited by activation of antioxidant enzymes by paracrine factors secreted by human placental multipotent mesenchymal stromal cells (hPMSC). hPMSC-conditioned medium and umbilical endothelial cells were assayed for cytokines and cytokine receptor expression by immunoassay and real-time PCR. Endothelial cell survival was evaluated by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] assay and caspase 3 activity assay. tert-Butyl hydroperoxide was used to induce oxidative injury in endothelial cells, with fluorescent microscopy and flow cytometry used to detect intracellular peroxides and cell apoptosis. Western blot, real-time PCR, STAT3 DNA-binding activity assay, and STAT3 siRNA were used to assess endothelial cell antioxidant enzymes. hPMSC-conditioned medium supported endothelial cell survival and reduced endothelial cell intracellular peroxides and apoptosis. hPMSCs expressed the transcripts of the interleukin (IL) 6 cytokine family, including IL6 and leukemia-i...Continue Reading

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Citations

Jul 9, 2013·The Journal of Surgical Research·Rajika RoyChristof Stamm
Mar 7, 2014·Pharmacology & Therapeutics·Annelies BronckaersIvo Lambrichts
Mar 14, 2014·Cell Adhesion & Migration·Chie-Pein Chen
Dec 19, 2012·Journal of Neuroimmunology·E BorysiewiczG W Konat
Oct 8, 2014·Cell Transplantation·Federico FranchiMartin Rodriguez-Porcel
Jan 1, 2013·Journal of Clinical Medicine·Sveva BolliniRanieri Cancedda
Jun 5, 2020·Stem Cells Translational Medicine·Rhian Stavely, Kulmira Nurgali
Feb 24, 2016·Free Radical Research·Preethi Sheshadri, Anujith Kumar
Aug 15, 2020·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Ling-Li JiXu Chen
Jan 22, 2021·Autoimmunity Reviews·Dominique FargeKarin Tarte

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Methods Mentioned

BETA
cesarean section
density gradient fractionation
immunoprecipitation
PCR
flow cytometry
fluorescence-activated cell sorting
Assay
transfection
ELISA
fluorescence microscopy

Software Mentioned

Cell Quest

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