Paracrine potential of adipose stromal vascular fraction cells to recover hypoxia-induced loss of cardiomyocyte function

Biotechnology and Bioengineering
Myroslava MytsykAnna Marsano

Abstract

Cell-based therapies show promising results in cardiac function recovery mostly through paracrine-mediated processes (as angiogenesis) in chronic ischemia. In this study, we aim to develop a 2D (two-dimensional) in vitro cardiac hypoxia model mimicking severe cardiac ischemia to specifically investigate the prosurvival paracrine effects of adipose tissue-derived stromal vascular fraction (SVF) cell secretome released upon three-dimensional (3D) culture. For the 2D-cardiac hypoxia model, neonatal rat cardiomyocytes (CM) were cultured for 5 days at < 1% (approaching anoxia) oxygen (O2 ) tension. Typical cardiac differentiation hallmarks and contractile ability were used to assess both the cardiomyocyte loss of functionality upon anoxia exposure and its possible recovery following the 5-day-treatment with SVF-conditioned media (collected following 6-day-perfusion-based culture on collagen scaffolds in either normoxia or approaching anoxia). The culture at < 1% O 2 for 5 days mimicked the reversible condition of hibernating myocardium with still living and poorly contractile CM (reversible state). Only SVF-medium conditioned in normoxia expressing a high level of the prosurvival hepatocyte-growth factor (HGF) and insulin-like growt...Continue Reading

References

Aug 26, 1998·International Journal of Cardiology·R FerrariO Visioli
Jan 18, 2006·Cardiovascular Research·Roberto J Diaz, Gregory J Wilson
Nov 11, 2008·Current Pharmaceutical Design·Giuseppe M C RosanoGiuseppe Barbaro
Jan 31, 2009·Nature Protocols·Nina TandonGordana Vunjak-Novakovic
Jun 17, 2009·Nature Reviews. Cardiology·Anja M van der LaanNiels van Royen
Aug 18, 2010·Journal of Molecular and Cellular Cardiology·Antoni Bayes-GenisJuan Carlos Izpisúa Belmonte
Sep 6, 2011·Journal of Molecular and Cellular Cardiology·John M Canty, Gen Suzuki
Mar 13, 2012·Lancet·Leon M PtaszekKenneth R Chien
Dec 22, 2012·Expert Opinion on Biological Therapy·Céline GiordanoMarc Ruel
Oct 10, 2013·Stem Cells Translational Medicine·Amanda J LeblancStuart K Williams
Apr 30, 2014·Nature Reviews. Cardiology·Fergus M O'Farrell, David Attwell
Jun 24, 2014·American Heart Journal·Emerson C PerinFrancisco Fernández-Avilés
Nov 13, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Lucio Díaz-FloresJuan F Madrid
Mar 5, 2016·Circulation Research·Kathleen M Broughton, Mark A Sussman
May 30, 2016·Methods in Molecular Biology·Massimiliano GnecchiMaria Chiara Ciuffreda
May 30, 2016·Methods in Molecular Biology·Patrizia DanieliMassimiliano Gnecchi
Mar 14, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Paola OcchettaAnna Marsano

❮ Previous
Next ❯

Citations

Sep 24, 2019·Expert Opinion on Biological Therapy·Isabel AndiaNatalia Burgos-Alonso
Oct 28, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Giuseppe IsuAnna Marsano

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.