Paracrine secretion of transforming growth factor-beta1 in aneurysm healing and stabilization with endovascular smooth muscle cell therapy

Journal of Vascular Surgery
Franck LosyEric Allaire

Abstract

Identification of molecular factors involved in artery wall stabilization after extracellular matrix injury elicited by inflammation and proteolysis has a major role in the development of new therapies for atherosclerosis. A study from our group demonstrated that endovascular seeding of vascular smooth muscle cells (VSMCs) promotes healing and stabilizes experimental aneurysms by downregulating matrix metalloproteinase and upregulating tissue inhibitor of metalloproteinase and collagen gene expression. We analyzed expression of transforming growth factor-beta (TGF-beta) and its receptors in experimental aneurysms treated with endovascular VSMC therapy. Aneurysms were generated in Fischer 344 rats by 14-day orthotopic implantation of a segment of guinea pig abdominal aorta (xenograft). During an endovascular repeat operation, syngeneic VSMCs were seeded in the aneurysm, always resulting in aneurysm diameter stabilization after 8 weeks, whereas diameter of control aneurysms infused with culture medium further increased. Seven days after repeat operation the intima or thrombus was separated from the aneurysmal wall in the two groups. Reverse transcriptase polymerase chain reaction with the domestic gene 18s as a standard demonstra...Continue Reading

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Citations

Jan 10, 2012·Tissue Engineering. Part B, Reviews·Chris A BashurAnand Ramamurthi
Feb 24, 2015·Stem Cells and Development·Mojtaba Parvizi, Martin C Harmsen
Jun 21, 2007·Seminars in Vascular Surgery·Matthew J Eagleton
Sep 3, 2014·Female Pelvic Medicine & Reconstructive Surgery·Bruna M CouriMargot S Damaser
Sep 7, 2017·Drug Delivery and Translational Research·Andrew CamardoAnand Ramamurthi
Nov 18, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Hualong BaiAlan Dardik
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Jan 29, 2013·Drug Delivery and Translational Research·Balakrishnan SivaramanAnand Ramamurthi
Apr 17, 2014·Journal of Tissue Engineering and Regenerative Medicine·Lavanya VenkataramanAnand Ramamurthi
Sep 29, 2011·Journal of Tissue Engineering and Regenerative Medicine·Chris A Bashur, Anand Ramamurthi

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