Paradoxical effects of ginkgolide B on cardiomyocyte contractile function in normal and high-glucose environments

Acta Pharmacologica Sinica
J KimJ Ren

Abstract

Ginkgo biloba extract is a natural product used widely for cerebral and cardiovascular diseases. It is mainly composed of terpene lactones (ginkgolide A and B) and flavone glycosides (eg quercetin and kaempferol). To better understand the cardiac electromechanical action of Ginkgo biloba extract in normal and diabetic states, this study was designed to examine the effect of ginkgolide B on cardiomyocyte contractile function under normal and high-glucose environments. Isolated adult rat ventricular myocytes were cultured for 6 h in a serum-free medium containing either normal (NG; 5.5 mmol/L) or high (HG; 25.5 mmol/L) glucose with or without ginkgolide B (0.5-2.0 microg/mL). Mechanical properties were evaluated using the IonOptix MyoCam system. Contractile properties analyzed included peak shortening (PS), maximal velocity of shortening/relengthening (+/-dl/dt), time-to-PS (TPS) and time-to-90% relengthening (TR90). Levels of essential Ca(2+) regulatory proteins sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA2a), phospholamban (PLB) and Na(+)-Ca(2+) exchanger (NCX) were assessed by Western blotting. Ginkgolide B nullified HG-induced prolongation in TR90. However, ginkgolide B depressed PS, +/-dl/dt and shortened TPS in NG and ...Continue Reading

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Citations

Jan 14, 2012·Journal of Cardiovascular Pharmacology·Giedre BaliutyteAdolfas Toleikis
Jun 25, 2010·American Journal of Physiology. Cell Physiology·Wai Ho TangStephen Sum Man Chung
Mar 13, 2009·High Altitude Medicine & Biology·Martha C Tissot van PatotPeter H Hackett

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