Paradoxical enhancement of oxidative cell injury by overexpression of heme oxygenase-1 in an anchorage-dependent cell ECV304

Journal of Cellular Biochemistry
Keiko MaruhashiAkihiro Yachie

Abstract

There has been increasing evidence suggesting the potent anti-inflammatory roles of heme oxygenase-1 (HO-1) in protecting renal tubular epithelial cells, vascular endothelial cells, and circulating monocytes. Based on these findings, novel therapeutic interventions have been proposed to control the expression of endothelial HO-1 levels to ameliorate various vascular diseases. We evaluated the effect of HO-1 gene transfer into an anchorage-dependent cell, ECV304. Effect of HO-1 production on the cell injury induced by hydrogen peroxide was evaluated after hemin stimulation and after HO-1 gene transfection. Morphological changes and the induction of various anti-apoptotic proteins were examined at the same time. Levels of HO-1 expression were variable in different clones of HO-1-transfected ECV304 cells. Among these, the clones with moderate levels of HO-1 expression were significantly more resistant to oxidative stress. In contrast, those with the highest levels of HO-1 exhibited paradoxically enhanced susceptibility to oxidative injury. Interestingly, the cell survival after oxidative stress was in parallel with the levels of Bcl-2 expression and of fibronectin receptor, alpha5 integrin. It is suggested from these results, that...Continue Reading

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Citations

Nov 6, 2007·Antioxidants & Redox Signaling·Nathalie Hill-KapturczakAnupam Agarwal
Mar 12, 2005·Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists·Kunio Ohta, Akihiro Yachie
Mar 3, 2007·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Akihiro Yachie
Dec 10, 2008·The International Journal of Biochemistry & Cell Biology·Marisol Orozco-IbarraJosé Pedraza-Chaverrí
Mar 29, 2008·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Aisling E Courtney, A Peter Maxwell
Oct 12, 2013·Pediatric Blood & Cancer·Taizo WadaAkihiro Yachie
Sep 9, 2008·Journal of Cellular and Molecular Medicine·Apriliana E R KartikasariDorine W Swinkels
Apr 21, 2007·Pediatrics International : Official Journal of the Japan Pediatric Society·Shoichi Koizumi
Mar 9, 2005·Critical Care Medicine·Hans-Georg Bone, Martin Westphal
Mar 19, 2016·Journal of Toxicologic Pathology·Yuko EmotoAiro Tsubura

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