Paralemmin-1 is over-expressed in estrogen-receptor positive breast cancers.

Cancer Cell International
Casey M TurkKathleen F Arcaro

Abstract

Paralemmin-1 is a phosphoprotein lipid-anchored to the cytoplasmic face of membranes where it functions in membrane dynamics, maintenance of cell shape, and process formation. Expression of paralemmin-1 and its major splice variant (Δ exon 8) as well as the extent of posttranslational modifications are tissue- and development-specific. Paralemmin-1 expression in normal breast and breast cancer tissue has not been described previously. Paralemmin-1 mRNA and protein expression was evaluated in ten breast cell lines, 26 primary tumors, and 10 reduction mammoplasty (RM) tissues using real time RT-PCR. Paralemmin-1 splice variants were assessed in tumor and RM tissues using a series of primers and RT-PCR. Paralemmin-1 protein expression was examined in cell lines using Western Blots and in 31 ductal carcinomas in situ, 65 infiltrating ductal carcinomas, and 40 RM tissues using immunohistochemistry. Paralemmin-1 mRNA levels were higher in breast cancers than in RM tissue and estrogen receptor (ER)-positive tumors had higher transcript levels than ER-negative tumors. The Δ exon 8 splice variant was detected more frequently in tumor than in RM tissues. Protein expression was consistent with mRNA results showing higher paralemmin-1 exp...Continue Reading

References

Mar 13, 2003·Breast Cancer Research : BCR·Sarah E BurdallValerie Speirs
Feb 6, 2004·Breast Cancer Research and Treatment·Marc Lacroix, Guy Leclercq
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Oct 27, 2010·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Eric DuncavageJohn Pfeifer

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Citations

Jan 21, 2014·Journal of Cellular Physiology·Katerina D Fagan-SolisKathleen F Arcaro

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Methods Mentioned

BETA
laser-capture microdissection
PCR
electrophoresis

Software Mentioned

Primer3
GraphPad Prism
GraphPad
PerlPrimer

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