PMID: 8948642Nov 15, 1996Paper

Parallel and antiparallel A*A-T intramolecular triple helices

Nucleic Acids Research
C DagneauxE Taillandier

Abstract

Intramolecular triple helices have been obtained by folding back twice oligonucleotides formed by decamers bound by non-nucleotide linkers: dA10-linker-dA10-linker-dT10 and dA10-linker-dT10-linker-dA10. We have thus prepared two triple helices with forced third strand orientation, respectively antiparallel (apA*A-T) and parallel (pA*A-T) with respect to the adenosine strand of the Watson-Crick duplex. The existence of the triple helices has been shown by FTIR, UV and fluorescence spectroscopies. Similar melting temperatures have been obtained in very different oligomer concentration conditions (micromolar solutions for thermal denaturation classically followed by UV spectroscopy, milimolar solutions in the case of melting monitored by FTIR spectroscopy) showing that the triple helices are intramolecular. The stability of the parallel triplex is found to be slightly lower than that of the antiparallel (deltaT(m) = 6 degrees C). The sugar conformations determined by FTIR are different for both triplexes. Only South-type sugars are found in the antiparallel triplex whereas both South- and North-type sugars are detected in the parallel triplex. In this case, thymidine sugars have a South-type geometry, and the adenosine strand of t...Continue Reading

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Citations

Dec 1, 1992·Journal of Biomolecular Structure & Dynamics·A AkhebatE Taillandier
Jan 16, 2002·Journal of Biomolecular Structure & Dynamics·J LiquierE Taillandier
Sep 24, 2008·Journal of Biomolecular Structure & Dynamics·Dmitry N KaluzhnyAnna K Shchyolkina
Aug 1, 1997·Antisense & Nucleic Acid Drug Development·C Giovannangeli, C Hélène
Mar 9, 2002·Nucleic Acids Research·Pedro Carmona, Marina Molina
Jul 11, 2006·Biophysical Chemistry·Arantxa Rodríguez-CasadoPedro Carmona
Apr 3, 2015·Organic & Biomolecular Chemistry·Tamer R KosbarErik B Pedersen
Apr 27, 1999·Electrophoresis·H PorumbE Taillandier
Oct 21, 2018·Journal of the American Society for Mass Spectrometry·Jiawei LiTara L Pukala
Feb 15, 2001·Oncogene·G K ScottC C Benz

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