Parallel cyclin E and cyclin A expression in neoplastic lesions of the uterine cervix

British Journal of Cancer
F ErlandssonA Zetterberg

Abstract

Cyclin E levels are high during late G1 and early S-phase in normal cells. The cyclin E expression over the cell cycle in tumours is not fully known. The impact on patient outcome by high cyclin E levels during other parts of the cell cycle than late G1- and early S-phase is unknown. We set out to study the expression of cyclin E over the cell cycle in cervical carcinomas. Using immunofluorescence staining of cyclin A, digital microscopy, and digital image analysis, we determined which cells in a tissue section that were in S- or G2-phase. M-phase cells were detected by morphology. By simultaneously staining for cyclin E, we investigated the variation in cyclin E levels over the cell cycle in cervical carcinoma lesions. In a case-control study, in which each deceased patient was matched with a patient still alive and well after >5 years of follow-up, we found that the deceased patients had a considerably higher fraction of cyclin A-positive cells staining for cyclin E than the survivors (n = 36). We conclude that parallel cyclin E and cyclin A expression is an indicator for poor outcome in cervical carcinomas. In addition, we investigated the expression pattern of cyclin E and cyclin A in consecutive biopsy samples from cervica...Continue Reading

References

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Citations

Jul 1, 2017·American Journal of Physical Anthropology·Catrine L JarmanBrian N Popp
Aug 3, 2013·Histopathology·Sultan Ahmad HalimiMasashi Fukayama
Jul 9, 2008·Acta Oncologica·Cecilia AhlinMarie-Louise Fjällskog

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Methods Mentioned

BETA
biopsy
biopsies
fluorescence microscopy
flow cytometry
chromosomal aberrations
transfection

Software Mentioned

IMP

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