Parallel detection of lactobacillus and bacterial vaginosis-associated bacterial DNA in the chorioamnion and vagina of pregnant women at term

The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
Sophia M R LannonDavid N Fredricks

Abstract

The majority of early preterm births are associated with intrauterine infections, which are thought to occur when microbes traffic into the uterus from the lower genital tract and seed the placenta. Bacterial vaginosis (BV) is associated with heterogeneous bacterial communities in the vagina and is linked to preterm birth. The extent to which trafficking into the uterus of normal and BV-associated vaginal bacteria occurs is unknown. The study objective was to characterize in parallel the distribution and quantities of bacteria in the vagina, uterus, and placental compartments. Pregnant women at term (≥37 weeks) presenting for delivery were recruited prospectively. Swabs were collected in parallel from the vagina, chorioamnion. Choriodecidual swabs were collected if a cesarean section was performed. Samples were analyzed by culture, broad-range 16S rRNA gene PCR, and bacterial species-specific quantitative PCR (qPCR) for DNA from Lactobacillus and a panel of BV-associated bacteria. Results were correlated with placental histopathology. Of the 23 women enrolled, 15 were delivered by cesarean section (N = 10 without labor; N = 5 in labor) and eight were delivered vaginally. BV was diagnosed in two women not in labor. Placental his...Continue Reading

References

Nov 1, 1995·American Journal of Obstetrics and Gynecology·M R JoesoefB Utomo
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S L HillierD A Eschenbach
Feb 13, 2001·Obstetrics and Gynecology·J HittiD A Eschenbach
Jan 22, 2005·Pediatric Research·Jennifer H SteelMark H F Sullivan
Feb 8, 2005·American Journal of Obstetrics and Gynecology·Mark A KlebanoffUNKNOWN National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
Sep 28, 2005·Current Microbiology·Esther JiménezJuan M Rodríguez
Nov 4, 2005·The New England Journal of Medicine·David N FredricksJeanne M Marrazzo
Jan 1, 1994·Infectious Diseases in Obstetrics and Gynecology·R S McDuffie, R S Gibbs
Oct 31, 2008·Journal of Clinical Microbiology·Yiping W HanCatalin S Buhimschi
Nov 13, 2008·Journal of Perinatal Medicine·Deborah B NelsonDavid N Fredricks
Dec 23, 2009·Obstetrics and Gynecology·Jean Pierre MenardFlorence Bretelle
Dec 6, 2011·Seminars in Fetal & Neonatal Medicine·Daniel B DiGiulio
May 4, 2012·Science Translational Medicine·Pawel GajerJacques Ravel
Jun 12, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karen RacicotGil Mor
Jan 11, 2014·Paediatric and Perinatal Epidemiology·Deborah B NelsonDavid N Fredricks
Mar 13, 2014·PloS One·Alexandria N ArdissoneJosef Neu
May 23, 2014·Science Translational Medicine·Kjersti AagaardJames Versalovic
Aug 16, 2014·Science·Roberto RomeroSusan J Fisher
Sep 19, 2014·Science Translational Medicine·Harvey J Kliman
Sep 19, 2014·Science Translational Medicine·Kjersti M Aagaard
Oct 2, 2015·Science Advances·Claire GendrinLakshmi Rajagopal
Jun 30, 2016·MBio·Jay VornhagenLakshmi Rajagopal
Feb 22, 2017·Frontiers in Cellular and Infection Microbiology·Vishesh KotharyJennifer A Gaddy
May 28, 2017·American Journal of Obstetrics and Gynecology·Molly J StoutMethodius G Tuuli
Sep 13, 2017·Scientific Reports·Lindsay A ParnellIndira U Mysorekar

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Citations

Sep 10, 2020·Frontiers in Cellular and Infection Microbiology·Kehinde S OlaniyiIrene Mackraj
Nov 17, 2019·Nature Reviews. Urology·Signe AltmäeReet Mändar
Apr 17, 2020·High-throughput·Rossella TomaiuoloValeria D'Argenio
Apr 8, 2021·Critical Reviews in Microbiology·Kevin R TheisNardhy Gomez-Lopez
Jun 5, 2021·Journal of Gynecology Obstetrics and Human Reproduction·Chuanfeng DingQian Zhou
Jun 13, 2021·Genomics, Proteomics & Bioinformatics·Zhuye JieHuijue Jia
Jul 27, 2021·The Journal of Allergy and Clinical Immunology·Yuan GaoPeter Vuillermin
Sep 25, 2021·Scientific Reports·Daichi UrushiyamaShingo Miyamoto
Oct 2, 2021·Scientific Reports·Jenni TurunenTerhi Tapiainen

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