Parallel DNA: generation of a duplex between two Drosophila sequences in vitro.

FEBS Letters
N A TchurikovY D Nechipurenko

Abstract

We have observed the existence of a parallel complementary region between two Drosophila DNA sequences, fragments of the suffix [(1986) EMBO J, 5, 2341-2347] and a 5'-non-coding sequence of the alcohol dehydrogenase gene [(1983) Cell 33, 125-133]. The region includes approximately 40 bp, 76% of which are complementary in the same polarity. Synthetic complementary 16 bp oligonucleotides corresponding to this region which were bound by the 5'-ends through a 1.6-hexanediol bridge form a duplex which displays both melting and annealing as judged by UV absorbance. Anti parallel complementary 16 bp long oligonucleotides bound by the 5'-3' ends through the same bridge and a single-strand sequence were used as controls. The Hoechst 33,258 drug binds to this parallel duplex of DNA; however, the properties of such a complex testify against the B-form of the duplex.

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Citations

Jan 23, 1992·Biochimica Et Biophysica Acta·J D ZhuB K Chernov
Dec 1, 1995·Journal of Biomolecular Structure & Dynamics·N A TchurikovB K Chernov
Jun 10, 1998·Journal of Biomolecular Structure & Dynamics·A K ShchyolkinaT M Jovin
Mar 28, 2002·Nucleic Acids Research·V Rani ParvathyH Todd Miles
Oct 14, 2008·Gene·Pradip Bhattacharya, Ramesh K Bamezai
Jun 5, 2013·Journal of Biomolecular Structure & Dynamics·Ol'ha O Brovarets'Dmytro M Hovorun
Oct 9, 1990·Biochemistry·M W GermannJ H van de Sande
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·E Palecek
Aug 5, 2017·The FEBS Journal·Marta Szabat, Ryszard Kierzek
Sep 17, 2011·Chemistry & Biodiversity·Piotr Guga, Maria Koziołkiewicz
Oct 21, 2003·Nucleosides, Nucleotides & Nucleic Acids·D N KaluzhnyT M Jovin
Mar 15, 2018·Frontiers in Chemistry·Ol'ha O Brovarets'Dmytro M Hovorun
Sep 1, 2000·Journal of Theoretical Biology·R Veitia, C Ottolenghi
Jun 1, 2010·Bioconjugate Chemistry·Akash K Jain, Santanu Bhattacharya
Jun 12, 2018·The Journal of Physical Chemistry. B·David ButcherFrancisco Fernandez-Lima

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