Parallel expression profiling of barley-stem rust interactions

Functional & Integrative Genomics
Ling ZhangA Kleinhofs

Abstract

The dominant barley stem rust resistance gene Rpg1 confers resistance to many but not all pathotypes of the stem rust fungus Puccinia graminis f. sp. tritici (Pgt). Transformation of Rpg1 into susceptible cultivar Golden Promise rendered the transgenic plants resistant to Pgt pathotype MCC but not to Pgt pathotype QCC. Our objective was to identify genes that are induced/repressed during the early stages of pathogen infection to elucidate the molecular mechanisms and role of Rpg1 in defense. A messenger ribonucleic acid expression analysis using the 22K Barley1 GeneChip was conducted in all pair-wise combinations of two isolines (cv. Golden Promise and Rpg1 transgenic line G02-448F-3R) and two Pgt pathotypes (MCC and QCC) across six time points. Analysis showed that a total of 34 probe sets exhibited expression pattern differences between Golden Promise (susceptible) and G02-448F-3R (resistant) infected with Pgt-MCC. A total of 14 probe sets exhibited expression pattern differences between Pgt-MCC (avirulent) and Pgt-QCC (virulent) inoculated onto G02-448F-3R. These differentially expressed genes were activated during the early infection process, before the hypersensitive response or fungal growth inhibition occurred. Our analy...Continue Reading

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Citations

Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·R BrueggemanA Kleinhofs
Jul 20, 2012·Molecular Plant-microbe Interactions : MPMI·Yan Meng, Roger P Wise
Jan 13, 2010·PloS One·Xinwei ChenRobbie Waugh
Aug 3, 2010·Journal of Plant Physiology·Melanie BischofRalph Hückelhoven
Oct 3, 2013·Biotechnology Advances·Katarína MrízováPetr Galuszka
Apr 10, 2017·Plant Cell Reports·Irini Nianiou-ObeidatNikolaos E Labrou
Nov 1, 2014·Plant Disease·L DerevninaR F Park

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