Parallel feedback loops control the basal activity of the HOG MAPK signaling cascade

Integrative Biology : Quantitative Biosciences From Nano to Macro
Hoda SharifianMatthias Peter

Abstract

Tight regulation of the MAP kinase Hog1 is crucial for survival under changing osmotic conditions. Interestingly, we found that Hog1 phosphorylates multiple upstream components, implying feedback regulation within the signaling cascade. Taking advantage of an unexpected link between glucose availability and Hog1 activity, we used quantitative single cell measurements and computational modeling to unravel feedback regulation operating in addition to the well-known adaptation feedback triggered by glycerol accumulation. Indeed, we found that Hog1 phosphorylates its activating kinase Ssk2 on several sites, and cells expressing a non-phosphorylatable Ssk2 mutant are partially defective for feedback regulation and proper control of basal Hog1 activity. Together, our data suggest that Hog1 activity is controlled by intertwined regulatory mechanisms operating with varying kinetics, which together tune the Hog1 response to balance basal Hog1 activity and its steady-state level after adaptation to high osmolarity.

References

Mar 19, 1993·Science·J L BrewsterM C Gustin
Dec 11, 2007·Journal of Proteome Research·Lukas KällWilliam Stafford Noble
Jan 26, 2008·Science·Jerome T MettetalAlexander van Oudenaarden
Mar 28, 2008·BMC Bioinformatics·Marc SturmOliver Kohlbacher
May 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Pascal HersenSharad Ramanathan
Aug 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick J WestfallJeremy Thorner
Aug 30, 2008·Molecular Biology of the Cell·David B Berry, Audrey P Gasch
Mar 26, 2009·Science Signaling·Javier MaciaFrancesc Posas
Jul 15, 2009·Cell·Dale MuzzeyAlexander van Oudenaarden
Nov 17, 2009·Nature Methods·Kohei NishimuraMasato Kanemaki
Sep 11, 2010·Nature·Ioannis LestasJohan Paulsson
Oct 5, 2010·Current Protocols in Molecular Biology·Arthur EdelsteinNico Stuurman
Sep 15, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Serge PeletMatthias Peter
Nov 15, 2012·Molecular Systems Biology·Jörg SchaberAlejandro Colman-Lerner
Dec 5, 2012·PLoS Computational Biology·Yihan LinNorbert F Scherer
Jun 12, 2013·Molecular Systems Biology·Bertram KlingerNils Blüthgen
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·J K EngJ R Yates
Feb 27, 2014·Science Signaling·Keiichiro TanakaHaruo Saito

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Citations

Oct 14, 2016·Frontiers in Microbiology·Ramon GonzalezEva Valero
Mar 9, 2017·Science Signaling·Natalie RomanovWolfgang Reiter
Mar 16, 2017·Molecular Microbiology·Miriam Rodríguez-GonzálezRoberto Coria
May 19, 2018·Frontiers in Cellular and Infection Microbiology·Ana Morales-MenchénRebeca Alonso-Monge
Jun 19, 2019·Cell Communication and Signaling : CCS·Marion JanschitzWolfgang Reiter
Jun 22, 2020·Current Genetics·Araceli Vázquez-IbarraRoberto Coria

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