Parallel Genomics Uncover Novel Enterococcal-Bacteriophage Interactions

MBio
Anushila ChatterjeeBreck A Duerkop

Abstract

Bacteriophages (phages) have been proposed as alternative therapeutics for the treatment of multidrug-resistant bacterial infections. However, there are major gaps in our understanding of the molecular events in bacterial cells that control how bacteria respond to phage predation. Using the model organism Enterococcus faecalis, we used two distinct genomic approaches, namely, transposon library screening and RNA sequencing, to investigate the interaction of E. faecalis with a virulent phage. We discovered that a transcription factor encoding a LytR family response regulator controls the expression of enterococcal polysaccharide antigen (epa) genes that are involved in phage infection and bacterial fitness. In addition, we discovered that DNA mismatch repair mutants rapidly evolve phage adsorption deficiencies, underpinning a molecular basis for epa mutation during phage infection. Transcriptomic profiling of phage-infected E. faecalis revealed broad transcriptional changes influencing viral replication and progeny burst size. We also demonstrate that phage infection alters the expression of bacterial genes associated with intra- and interbacterial interactions, including genes involved in quorum sensing and polymicrobial compet...Continue Reading

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Jun 14, 2020·Nature Reviews. Genetics·Amy K CainTim van Opijnen
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Datasets Mentioned

BETA
E-MTAB-8546
PRJEB35492

Methods Mentioned

BETA
Infection
PCR
phosphotransferase
RNA-Seq

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