Parallel isotope differential modeling for instationary 13C fluxomics at the genome scale

Biotechnology for Biofuels
Zhengdong ZhangXiaoyao Xie

Abstract

A precise map of the metabolic fluxome, the closest surrogate to the physiological phenotype, is becoming progressively more important in the metabolic engineering of photosynthetic organisms for biofuel and biomass production. For photosynthetic organisms, the state-of-the-art method for this purpose is instationary 13C fluxomics, which has arisen as a sibling of transcriptomics or proteomics. Instationary 13C data processing requires solving high-dimensional nonlinear differential equations and leads to large computational and time costs when its scope is expanded to a genome-scale metabolic network. Here, we present a parallelized method to model instationary 13C labeling data. The elementary metabolite unit (EMU) framework is reorganized to allow treating individual mass isotopomers and breaking up of their networks into strongly connected components (SCCs). A variable domain parallel algorithm is introduced to process ordinary differential equations in a parallel way. 15-fold acceleration is achieved for constant-step-size modeling and ~ fivefold acceleration for adaptive-step-size modeling. This algorithm is universally applicable to isotope granules such as EMUs and cumomers and can substantially accelerate instationary ...Continue Reading

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Software Mentioned

hfill
COBRApy
FiatFlux
ConcurrentHashMap
imSyn593
INCA
13CFLUX2
WUFlux
OptGPSampler
OpenMebius

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