Parallel labeling experiments for pathway elucidation and (13)C metabolic flux analysis

Current Opinion in Biotechnology
Maciek R Antoniewicz

Abstract

Metabolic pathway models provide the foundation for quantitative studies of cellular physiology through the measurement of intracellular metabolic fluxes. For model organisms metabolic models are well established, with many manually curated genome-scale model reconstructions, gene knockout studies and stable-isotope tracing studies. However, for non-model organisms a similar level of knowledge is often lacking. Compartmentation of cellular metabolism in eukaryotic systems also presents significant challenges for quantitative (13)C-metabolic flux analysis ((13)C-MFA). Recently, innovative (13)C-MFA approaches have been developed based on parallel labeling experiments, the use of multiple isotopic tracers and integrated data analysis, that allow more rigorous validation of pathway models and improved quantification of metabolic fluxes. Applications of these approaches open new research directions in metabolic engineering, biotechnology and medicine.

References

Oct 27, 2006·Metabolomics : Official Journal of the Metabolomic Society·Maciek R AntoniewiczJoanne K Kelleher
Nov 8, 2006·Metabolic Engineering·Maciek R AntoniewiczGregory Stephanopoulos
Sep 8, 2007·Analytical Chemistry·Maciek R AntoniewiczGregory Stephanopoulos
Feb 20, 1995·Biotechnology and Bioengineering·C Zupke, G Stephanopoulos
Sep 2, 2008·Applied Microbiology and Biotechnology·Joungmin LeeSang Yup Lee
Sep 4, 2008·Biotechnology and Bioengineering·Ryan S Senger, Eleftherios T Papoutsakis
Feb 19, 2010·Current Opinion in Biotechnology·Jens NiklasElmar Heinzle
Aug 26, 2010·The Journal of Biological Chemistry·Charlotte E M NunnMichael J Danson
Sep 3, 2010·The Journal of Biological Chemistry·Kuo-Hsiang TangYinjie J Tang
Sep 18, 2010·Bioprocess and Biosystems Engineering·Olivier HenryAmine Kamen
Oct 13, 2010·The Journal of Biological Chemistry·Xueyang FengYinjie J Tang
Feb 18, 2011·Biotechnology and Bioengineering·Prabhasa RavikirthiCostas D Maranas
Sep 13, 2011·Biotechnology Journal·Judith WahrheitElmar Heinzle
Nov 22, 2011·Biotechnology Journal·Woo Suk Ahn, Maciek R Antoniewicz
Dec 27, 2011·Metabolic Engineering·Jason L WaltherGregory Stephanopoulos
May 18, 2012·BMC Systems Biology·Scott B CrownMaciek R Antoniewicz
Aug 17, 2012·Journal of the Royal Society, Interface·Joseph Kuo-Hsiang TangYinjie J Tang
Nov 13, 2012·Current Opinion in Biotechnology·Maciek R Antoniewicz
Dec 5, 2012·Current Opinion in Biotechnology·Karsten Hiller, Christian M Metallo
Dec 19, 2012·Metabolic Engineering·Scott B Crown, Maciek R Antoniewicz
Feb 12, 2013·Molecular Cell·Christian M Metallo, Matthew G Vander Heiden
Mar 5, 2013·Current Opinion in Biotechnology·Maciek R Antoniewicz
May 18, 2013·Biotechnology Advances·Yoshihiro Toya, Hiroshi Shimizu
Jun 4, 2013·Current Opinion in Biotechnology·Jamey D Young
Sep 12, 2013·Metabolic Engineering·Robert W Leighty, Maciek R Antoniewicz
Sep 13, 2013·Metabolic Engineering·Scott B Crown, Maciek R Antoniewicz
Feb 25, 2014·Current Opinion in Biotechnology·Björn H Junker
Mar 19, 2014·Current Opinion in Biotechnology·Rajib SahaCostas D Maranas
Mar 19, 2014·Current Opinion in Biotechnology·Joonhoon Kim, Jennifer L Reed
Apr 2, 2014·Current Opinion in Biotechnology·Christopher P Long, Maciek R Antoniewicz
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Wei-Qin ZhuangLisa Alvarez-Cohen
May 9, 2014·Nature·Jing FanJoshua D Rabinowitz
Jun 3, 2014·Molecular Cell·Caroline A LewisChristian M Metallo
Dec 3, 2014·Molecular Cell·Nathaniel M VacantiChristian M Metallo

❮ Previous
Next ❯

Citations

Oct 26, 2016·Current Opinion in Biotechnology·Jacqueline E Gonzalez, Maciek R Antoniewicz
Aug 9, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Scott B CrownMaciek R Antoniewicz
Apr 17, 2018·Experimental & Molecular Medicine·Maciek R Antoniewicz
Jun 11, 2019·Frontiers in Microbiology·Martin BeyßKatharina Nöh
Sep 1, 2019·Nature Protocols·Christopher P Long, Maciek R Antoniewicz
Feb 13, 2020·Chemical Communications : Chem Comm·Shohei UchinomiyaAkio Ojida
Feb 9, 2019·Frontiers in Microbiology·Jungik Choi, Maciek R Antoniewicz
Aug 8, 2019·Metabolic Engineering·Christopher P Long, Maciek R Antoniewicz
Mar 10, 2018·Current Opinion in Biotechnology·Yi Ern Cheah, Jamey D Young
Jul 28, 2020·Current Opinion in Biotechnology·Maciek R Antoniewicz
Mar 18, 2020·Metabolic Engineering·R Kyle BennettEleftherios T Papoutsakis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

Journal of Proteome Research
Yoshihiro ToyaMasaru Tomita
Bioinformatics
Katharina NöhWolfgang Wiechert
Methods in Molecular Biology
Maciek R Antoniewicz
© 2021 Meta ULC. All rights reserved