PMID: 3754001Mar 1, 1986Paper

Parallel postnatal development of choline acetyltransferase activity and muscarinic acetylcholine receptors in the rat olfactory bulb

Journal of Neurochemistry
T H LargeW L Klein

Abstract

The development of cholinergic synapses in the rat olfactory bulb was investigated by measuring changes in the activity of choline acetyltransferase (ChAT; EC 2.3.1.6.), a presynaptic cholinergic marker, and in the concentration of muscarinic receptors, components of cholinoceptive membranes. Three biochemical properties of the muscarinic system also were examined for possible differentiation: ligand binding, molecular weight, and isoelectric point. Receptors from embryonic (day 18), neonatal (postnatal day 3), and adult rat olfactory bulbs exhibited identical complex binding (nH = 0.45) of the agonist carbachol. For each age, the relative proportions of high-affinity (Ki approximately equal to 1.0 microM) and low-affinity (Ki approximately equal to 100 microM) binding states were 60% and 40%, respectively. The antagonist pirenzepine also bound to high-affinity (Ki approximately equal to 0.15 microM, RH approximately equal to 70%) and low-affinity (Ki approximately equal to 2.0 microM, RL approximately equal to 30%) sites in neonatal and adult rats. Sodium dodecyl sulfate/urea-polyacrylamide gel electrophoresis of [3H]propylbenzilylcholine mustard-labeled receptors from neonatal and adult rats showed a single electrophoretic fo...Continue Reading

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Dec 1, 1991·Brain Research Bulletin·E GouldL L Butcher
Jan 1, 1990·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E P FiedlerA C Collins
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Apr 1, 1992·Journal of Neurobiology·M MartinicW L Klein

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